Nws ua haujlwm los ntawm tsim cov spectrum los ntawm kev sib cais ions ntawm qhov sib txawv ntawm qhov sib txawv rau tus nqi sib piv (m / z) qhov twg m yog molecular lossis atomic mass, z yog lub electrostatic charge unit. … Tandem huab hwm coj spectrometer yog ib qho cuab yeej siv ob (lossis ntau dua) cov ntsuas huab cua.
Vim li cas tandem huab hwm coj spectrometry siv?
Tandem huab hwm coj spectrometry tuaj yeem tsim cov peptide ib ntus tag uas tuaj yeem siv los txheeb xyuas peptide hauv cov ntaub ntawv protein… Lwm cov fragment ions yuav raug soj ntsuam nyob rau hauv siab zog dissociation tej yam kev mob.
Tandem chromatography yog dab tsi?
GC-MS/MS. Gas chromatography yog cov txheej txheem sib cais rau cov tsis muaj zog lossis tsis muaj polar compounds. Tandem huab hwm coj spectrometry yog cov txheej txheem tshawb nrhiav uas ionizes thiab tawg moleculesOb qho tib si ua ntej thiab cov khoom lag luam ions raug ntsuas uas ua rau nce qhov tshwj xeeb thiab kev xaiv ntawm cov txheej txheem tshawb pom no.
Tandem MS ua haujlwm li cas hauv cov txheej txheem peptide sequencing?
Tandem MS Sib tsoo-Activated Dissociation
MS / MS, txheej txheem ntawm peptide ion fragmentation nrog kev ntsuas m / z tom ntej, feem ntau induced los ntawm kev cais cov protonated peptide m / z ntawm txaus siab thiab ua rau nws ntau pua kev sib tsoo nrog cov pa roj tsawg tsawg(5).
Txoj cai ntawm LC-MS yog dab tsi?
Lub LC-MS thev naus laus zis suav nrog kev siv HPLC, qhov twg cov khoom sib xyaw hauv ib qho sib xyaw yog thawj zaug sib cais tom qab ionization thiab kev sib cais ntawm cov ions raws li lawv qhov loj / nqi piv.
