Cov hauv qab no yog ib qho kev piav qhia ntawm cov kauj ruam sib txawv ntawm kev tiv thaiv kab mob tsis ncaj ncees lawm
- Kev Npaj Ua Haujlwm thiab Kev Npaj Ua Qauv. …
- Sample Fixation. …
- Cell Permeabilization. …
- Kev thaiv. …
- Primary Antibody Incubation. …
- Secondary Antibody Incubation. …
- Txoj kev thiab Mounting.
Koj ua li cas immunofluorescence?
Txhua cov kauj ruam incubation tshwm sim ntawm chav tsev kub
- Ntxuav cov hlwb ob zaug thiab siv cov tweezers kom ua tib zoo tso cov ntaub npog nrog cov cell upturned rau hauv lub chamber humidified.
- Kho nrog 4% formaldehyde rau 10 feeb thiab ntxuav 3 ×.
- Permeabilize nrog 0.1 % TX-100 / PBS rau 15-20 feeb thiab ntxuav 3 ×.
Koj siv immunofluorescence hauv lub cell li cas?
Immunofluorescence raws tu qauv rau cov hlwb adherent
- Noob 1–1.5 x104 hlwb ib qhov dej ntawm 4-chamber swb hauv 500 mL ntawm kab lis kev cai nruab nrab. Muab tso rau ntawm 37 ° C ntawm 5% CO2.
- 32–36 teev tom qab cov noob qoob loo, tshem tawm cov kab lis kev cai nruab nrab ntawm tes thiab yaug cov hlwb 3 zaug siv 500 µL ntawm 1X PBS.
piv txwv ntawm immunofluorescence yog dab tsi?
Piv txwv li, tus kws tshawb fawb yuav tsim cov tshuaj tiv thaiv thawj zaug hauv tshisuas paub txog ntau yam antigens, thiab tom qab ntawd siv cov tshuaj zas-coupled luav cov tshuaj tiv thaiv kab mob thib ob uas paub txog cov tshis tshuaj tiv thaiv thaj tsam tas li (" luav anti-tshis" antibodies).
Kev siv tshuaj tiv thaiv kab mob fluorescence yog dab tsi?
capunofluorescence (yog tias) staining yog ib tug dav siv cov txheej txheem nyob rau hauv kev tshawb fawb txog roj ntsha hauv kev txiav txim siab txhawm rau txhawm rau txhawm rau paub cov hom phiaj anigens. Ua raws li kev kos duab, nws yog cov txheej txheem ncaj qha raws li koj tuaj yeem pom qee yam.
