Cov txheej txheem:
- Cov hauv qab no yog ib qho kev piav qhia ntawm cov kauj ruam sib txawv ntawm kev tiv thaiv kab mob tsis ncaj ncees lawm
- Koj ua li cas immunofluorescence?
- Koj siv immunofluorescence hauv lub cell li cas?
- piv txwv ntawm immunofluorescence yog dab tsi?
- Kev siv tshuaj tiv thaiv kab mob fluorescence yog dab tsi?
Video: Yuav ua li cas ua immunofluorescence?
2024 Tus sau: Fiona Howard | [email protected]. Kawg hloov kho: 2024-01-10 06:38
Cov hauv qab no yog ib qho kev piav qhia ntawm cov kauj ruam sib txawv ntawm kev tiv thaiv kab mob tsis ncaj ncees lawm
- Kev Npaj Ua Haujlwm thiab Kev Npaj Ua Qauv. …
- Sample Fixation. …
- Cell Permeabilization. …
- Kev thaiv. …
- Primary Antibody Incubation. …
- Secondary Antibody Incubation. …
- Txoj kev thiab Mounting.
Koj ua li cas immunofluorescence?
Txhua cov kauj ruam incubation tshwm sim ntawm chav tsev kub
- Ntxuav cov hlwb ob zaug thiab siv cov tweezers kom ua tib zoo tso cov ntaub npog nrog cov cell upturned rau hauv lub chamber humidified.
- Kho nrog 4% formaldehyde rau 10 feeb thiab ntxuav 3 ×.
- Permeabilize nrog 0.1 % TX-100 / PBS rau 15-20 feeb thiab ntxuav 3 ×.
Koj siv immunofluorescence hauv lub cell li cas?
Immunofluorescence raws tu qauv rau cov hlwb adherent
- Noob 1–1.5 x104 hlwb ib qhov dej ntawm 4-chamber swb hauv 500 mL ntawm kab lis kev cai nruab nrab. Muab tso rau ntawm 37 ° C ntawm 5% CO2.
- 32–36 teev tom qab cov noob qoob loo, tshem tawm cov kab lis kev cai nruab nrab ntawm tes thiab yaug cov hlwb 3 zaug siv 500 µL ntawm 1X PBS.
piv txwv ntawm immunofluorescence yog dab tsi?
Piv txwv li, tus kws tshawb fawb yuav tsim cov tshuaj tiv thaiv thawj zaug hauv tshisuas paub txog ntau yam antigens, thiab tom qab ntawd siv cov tshuaj zas-coupled luav cov tshuaj tiv thaiv kab mob thib ob uas paub txog cov tshis tshuaj tiv thaiv thaj tsam tas li (" luav anti-tshis" antibodies).
Kev siv tshuaj tiv thaiv kab mob fluorescence yog dab tsi?
capunofluorescence (yog tias) staining yog ib tug dav siv cov txheej txheem nyob rau hauv kev tshawb fawb txog roj ntsha hauv kev txiav txim siab txhawm rau txhawm rau txhawm rau paub cov hom phiaj anigens. Ua raws li kev kos duab, nws yog cov txheej txheem ncaj qha raws li koj tuaj yeem pom qee yam.
Pom zoo:
Yuav ua li cas ib tug noog yuav tsaws saum dej?
Herons tsaws ib ncig ntawm lub pas dej thiab taug kev mus rau dej. Ua rau lawv nrog dej. … Herons yog cov tsiaj nyob ib leeg thiab nyiam noj ib leeg ntau dua li ntawm lwm tus herons . Can herons tsaws hauv pas dej? Lawv nyiam mus yos hav zoov hauv dej, qhov chaw uas lawv nyob twj ywm, ua ntej muab lawv lub caj dab mus rau hauv dej thiab nqos ntses tag.
Yuav ua li cas txoj kev sib yuav ntawm menyuam yaus yuav tsum raug tshem tawm?
Cov neeg sib tw hais tias xaus kev sib yuav me nyuam yuav txhawb kev ua kom tiav Cov Hom Phiaj los ntawm kev txhim kho kev nkag mus rau kev kawm, txhawb kev loj hlob ntawm kev lag luam, txhawb kev noj zaub mov zoo thiab kev ruaj ntseg zaub mov, thiab txhim kho kev noj qab haus huv ntawm niam thiab menyuam.
Yuav tsum taug kev coj tus cwj pwm yuav raug cai vim li cas lossis vim li cas ho tsis?
Cov neeg siv khoom yuav tsum muaj peev xwm tau txais lawv cov ntaub ntawv tus kheej lossis tus cwj pwm coj los ntawm ib lub koom haum koom nrog kev taug qab lossis kev tsom. Txhua lub koom haum koom nrog hauv ib qho kev coj tus cwj pwm los yog kev tsom mus rau kev ua haujlwm yuav tsum muaj lub luag haujlwm rau kev ua raws li txoj cai thiab nws cov cai.
Yuav ua li cas thiaj txo cov kab mob keeb kwm yav dhau hauv immunofluorescence?
Txog kom tsis txhob muaj cov tshuaj tiv thaiv kab mob tshwj xeeb khi thiab, vim li ntawd, txo cov teeb liab tom qab, nws raug nquahu kom siv cov tshuaj tiv thaiv ua ntej incubation nrog cov tshuaj tiv thaivCov tshuaj tiv thaiv zoo tshaj plaws yuav yog cov uas muaj cov ntshav los ntawm tib hom uas cov tshuaj tiv thaiv kab mob thib ob tau tsa .
Yuav sau li cas vim li cas thiab yog vim li cas?
Kev txhais ntawm yog vim li cas thiab (tus) yog li ntawd: yog vim li cas rau ib yam Nws piav qhia vim li cas thiab yog li ntawd ntawm tus nqi nce . Yuav ua li cas thiab yog vim li cas? Txhua qhov laj thawj thiab qhov laj thawj, ib yam li hauv Nws tau mus rau qhov laj thawj thiab yog li ntawm lub koom haum kev saws me nyuam txoj cai thiab cov txheej txheem.
