Txog kom tsis txhob muaj cov tshuaj tiv thaiv kab mob tshwj xeeb khi thiab, vim li ntawd, txo cov teeb liab tom qab, nws raug nquahu kom siv cov tshuaj tiv thaiv ua ntej incubation nrog cov tshuaj tiv thaivCov tshuaj tiv thaiv zoo tshaj plaws yuav yog cov uas muaj cov ntshav los ntawm tib hom uas cov tshuaj tiv thaiv kab mob thib ob tau tsa.
Koj ua li cas tiv thaiv keeb kwm yav dhau?
Txo qhov tuab ntawm cov ntaub so ntswgtuaj yeem pab txo qis tom qab staining. Sim txiav cov ntu nyias nyias rau kev huv IHC. Nws kuj tseem ceeb heev uas yuav tsum tau saib xyuas kom tsis txhob cia cov ntu qhuav tawm vim tias ziab tuaj yeem ua rau muaj qhov tsis muaj qhov tshwj xeeb.
Koj ua li cas kho cov kab mob immunofluorescence?
Immunofluorescence Microscopy: 10 Lub Tswv Yim rau Kev Ua Tau Zoo
- Cell fixation thiab Permeabilization. …
- Antibody Specificity. …
- Siv cov tshuaj tiv thaiv kab mob kom tsim nyog. …
- Txhim kho qhov tsis zoo thiab thaiv tus neeg sawv cev. …
- Siv qhov tsim nyog Cell Ceev. …
- Ntau Staining. …
- Secondary Antibodies. …
- Qhia keeb kwm yav dhau.
Dab tsi ua rau tom qab staining?
Cov xim tom qab tuaj yeem tshwm sim los ntawm tsis tsim nyog cov tshuaj tiv thaiv kab mob los yog ua yuam kev thaum npaj cov ntaub so ntswg.
Pob cov tshuaj tiv thaiv theem nrab txo cov cim keeb kwm yav dhau?
F(ab) cov tshuaj tiv thaiv kab mob thib ob tuaj yeem pab txo cov teeb meem keeb kwm yav dhau los ntawm tshem tawm cov kev khi tsis tshwj xeeb ntawmcov IgG cov proteins pom hauv cov ntaub so ntswg los ntawm cov tshuaj tiv thaiv thib ob.
