Kev kuaj pom cov proteins carbonylated raws li kev sib cais electrophoretic tuaj yeem ua tiav ob qho tib si los ntawm Western blot thiab los ntawm in-gel fluorophoric tagging - ib qho kev pheej yig dua - nrog cov txiaj ntsig zoo sib xws.
Yuav ntsuas cov protein carbonylation li cas?
Protein carbonylation yog qhov kev siv ntau tshaj plaws ntawm kev hloov kho oxidative ntawm cov proteins. Nws yog feem ntau ntsuas spectrophotometrically lossis immunochemically los ntawm derivatizing proteins nrog cov classical carbonyl reagent 2, 4 dinitrophenylhydrazine (DNPH).
protein carbonylation yog dab tsi?
Protein carbonylation yog ib hom protein oxidation uas tuaj yeem txhawb nqa los ntawm cov pa oxygen reactive. Nws feem ntau yog hais txog cov txheej txheem uas tsim cov ketones reactive lossis aldehydes uas tuaj yeem cuam tshuam los ntawm 2, 4-dinitrophenylhydrazine (DNPH) los tsim hydrazones.
Dab tsi ua rau cov protein oxidation?
Protein oxidation yog txhais raws li covalent hloov pauv ntawm cov protein tshwm sim los ntawm ncaj qha cuam tshuam nrog cov pa oxygen reactive (ROS)lossis kev tsis ncaj ncees nrog cov khoom thib ob ntawm oxidative kev ntxhov siab.
Cov tshuaj tiv thaiv carbonylation yog dab tsi?
Carbonylation yog hais txog rau cov tshuaj tiv thaiv uas qhia cov pa roj carbon monoxide rau hauv cov organic thiab inorganic substrates Ntau cov tshuaj organic uas muaj txiaj ntsig zoo yog npaj los ntawm carbonylations, uas tuaj yeem xaiv cov tshuaj tiv thaiv zoo heev. Carbonylations tsim cov organic carbonyls, piv txwv li, cov tebchaw uas muaj C=O.
