Cov txheej txheem:
- YYuav ua li cas tshem tawm cov protein?
- Vim li cas cov proteins muaj pH elution qis?
- Yuav ua li cas elution ua tiav?
- Koj xav tau pes tsawg cov tshuaj tiv thaiv rau Coip?
Video: Koj puas tuaj yeem hais cov protein?
2024 Tus sau: Fiona Howard | [email protected]. Kawg hloov kho: 2023-12-16 01:49
Yog tias koj cov protein tsis zoo ib yam li koj cov tshuaj tiv thaiv kab mob, koj tuaj yeem elute nrog 0.1M glycine, pH2-3 thiab tom qab ntawd cais cov protein ntawm kev txaus siab los ntawm cov tshuaj tiv thaiv siv SEC (zoo li superdex 200).
YYuav ua li cas tshem tawm cov protein?
Cov proteins yog eluted los ntawm a gradient ntawm nce nqi ntawm cov organic solvent, xws li acetonitrile. Cov proteins elute raws li lawv cov hydrophobicity. Tom qab ntxuav los ntawm HPLC cov protein yog nyob rau hauv ib tug tov uas tsuas muaj volatile tebchaw, thiab tau yooj yim lyophilized.
Vim li cas cov proteins muaj pH elution qis?
Kev siv cov pH tsis tshua muaj rau elution los ntawm cov protein A yog paub los pab txhawb rau cov khoom sib sauTxawm li cas los xij, ib qho kev txwv ntau ntawm cov pov thawj qhia tias pH qis yuav tsis yog ib leeg. ua rau kev sib sau ua ke hauv cov protein A chromatography, theej, lwm yam ntawm cov txheej txheem yuav ua rau muaj txiaj ntsig zoo.
Yuav ua li cas elution ua tiav?
Nyob rau hauv analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent; raws li nyob rau hauv kev ntxuav ntawm loaded ion-exchange resins kom tshem tawm ions ntes. … Tom qab cov kuab tshuaj molecules tshem tawm cov analytes, cov analyte tuaj yeem nqa tawm ntawm kem rau kev tsom xam.
Koj xav tau pes tsawg cov tshuaj tiv thaiv rau Coip?
Rau cov kev sim Co-IP niaj hnub, cov tshuaj tiv thaiv uas kuv siv yog tsis ntau tshaj 2ug (Hauv kuv teeb, 1.4 -2.0ug ntawm cov tshuaj tiv thaiv yog txaus rau kev ntes 2500-5000ug ntawm protein lysate.) Tab sis nws tseem nyob ntawm qhov qhia theem ntawm koj lub hom phiaj proteins hauv koj cov qauv, yog li koj yuav tau ua qee qhov kev hloov kho.
How Much Protein Do You Need? Explained by Dr. Berg
Pom zoo:
Puas kaw cov hmoov protein puas tuaj yeem ua phem?
Cov lus teb yog yog, cov hmoov protein tsis tas sij hawm Txawm tias cov hmoov protein - yog tias khaws cia kom zoo - tsis tas sij hawm zoo li cov nqaij thiab cov khoom tshiab, nws tuaj yeem ua rau tsis zoo. Ib yam li cov ntim khoom zoo, cov hmoov protein muaj hnub tas sij hawm, uas yuav tsum tau luam tawm qhov chaw ntawm lub thawv .
Koj puas tuaj yeem hais tsis hais txog?
Yog ib yam dab tsi ua tiav yam tsis xav txog, nws ua tiav txawm li cas los xij, feem ntau ua raws li lo lus "ntawm." Hnub no koj tuaj yeem zaum txhua qhov chaw koj xav tau tsheb npav, tsis hais koj haiv neeg twg, tab sis qhov no tsis yog ib txwm muaj .
Koj puas tuaj yeem siv cov kab lus hais tawm sab nraud?
Yog koj tuaj yeem nrog Command™ Cov Khoom Sab Nraud. Cov nplaum yuav tsum tau siv rau qhov chaw huv, du, qhuav thaum qhov kub siab tshaj 15 ° F (-9 ° C). Cov khoom tuav tau qis qis rau -20 ° F (-29 ° C) . Puas yog cov lus txib tsis tu ncua tsis muaj dej?
Koj puas tuaj yeem siv cov lus hais hauv kab lus?
Recidivism kab lus piv txwv Txawm tias muaj kev ua txhaum cai (feem pua ntawm cov neeg saib xyuas yog cov neeg ua txhaum cai qub) tus nqi recidivism zero. Recommittals tau nquag thiab recidivism ntawm qhov nce. Peb tau nkag mus rau hauv txoj haujlwm tsis tuaj yeem sib cav tias kev rov ua dua yog ib qho kev cuam tshuam nkaus xwb .
Koj lub cev puas tuaj yeem tsim cov leeg tsis muaj protein?
"Ib feem ntau ntawm koj cov calorie ntau ntau yuav tsum yog los ntawm cov khoom noj uas muaj protein ntau, uas yuav muab cov amino acids tsim nyog rau koj los tsim cov leeg nqaij. Yog tsis muaj cov protein, koj tsuas yog nce rog thiab nqaij me xwb "
