Yog tias koj cov protein tsis zoo ib yam li koj cov tshuaj tiv thaiv kab mob, koj tuaj yeem elute nrog 0.1M glycine, pH2-3 thiab tom qab ntawd cais cov protein ntawm kev txaus siab los ntawm cov tshuaj tiv thaiv siv SEC (zoo li superdex 200).
YYuav ua li cas tshem tawm cov protein?
Cov proteins yog eluted los ntawm a gradient ntawm nce nqi ntawm cov organic solvent, xws li acetonitrile. Cov proteins elute raws li lawv cov hydrophobicity. Tom qab ntxuav los ntawm HPLC cov protein yog nyob rau hauv ib tug tov uas tsuas muaj volatile tebchaw, thiab tau yooj yim lyophilized.
Vim li cas cov proteins muaj pH elution qis?
Kev siv cov pH tsis tshua muaj rau elution los ntawm cov protein A yog paub los pab txhawb rau cov khoom sib sauTxawm li cas los xij, ib qho kev txwv ntau ntawm cov pov thawj qhia tias pH qis yuav tsis yog ib leeg. ua rau kev sib sau ua ke hauv cov protein A chromatography, theej, lwm yam ntawm cov txheej txheem yuav ua rau muaj txiaj ntsig zoo.
Yuav ua li cas elution ua tiav?
Nyob rau hauv analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent; raws li nyob rau hauv kev ntxuav ntawm loaded ion-exchange resins kom tshem tawm ions ntes. … Tom qab cov kuab tshuaj molecules tshem tawm cov analytes, cov analyte tuaj yeem nqa tawm ntawm kem rau kev tsom xam.
Koj xav tau pes tsawg cov tshuaj tiv thaiv rau Coip?
Rau cov kev sim Co-IP niaj hnub, cov tshuaj tiv thaiv uas kuv siv yog tsis ntau tshaj 2ug (Hauv kuv teeb, 1.4 -2.0ug ntawm cov tshuaj tiv thaiv yog txaus rau kev ntes 2500-5000ug ntawm protein lysate.) Tab sis nws tseem nyob ntawm qhov qhia theem ntawm koj lub hom phiaj proteins hauv koj cov qauv, yog li koj yuav tau ua qee qhov kev hloov kho.
How Much Protein Do You Need? Explained by Dr. Berg
