SDS ruaj khov rau qhov zoo them thiab cov hydrophobic residues ntawm cov proteins los ntawm nws pawg sulfate thiab alkyl chains , feem 13Raws li, Nws yog ib qho tshuaj ntxuav tes muaj txiaj ntsig hauv kev ua haujlwm ntawm cov txheej txheem biology thiab protein folding / unfolding kev tshawb fawb.
SDS ua li cas rau cov protein?
SDS yog ib qho amphipathic surfactant. Nws denatures proteins los ntawm kev khi rau cov saw hlau nrog nws cov hydrocarbon tail, nthuav tawm cov cheeb tsam ib txwm faus thiab txheej txheej cov saw hlau nrog cov molecules surfactant. Cov pab pawg neeg polar taub hau ntawm SDS ntxiv qhov txiaj ntsig ntxiv rau kev siv cov denaturant no.
SDS khi rau cov amino acids li cas?
SDS muaj tus Tsov tus tw hydrophobic uas cuam tshuam nrog cov protein (polypeptide) chains. Tus naj npawb ntawm SDS molecules uas khi rau ib qho protein yog feem ntau rau cov amino acids uas tsim cov proteinTxhua SDS molecule pab txhawb ob qhov tsis zoo, dhau los ntawm cov nqi protein yuav muaj.
Puas SDS lub tsho tiv no cov proteins?
SDS kuj coats cov protein nrog cov nqi tsis zoo, uas npog cov nqi tsim nyog ntawm R-pab pawg. SDS khi ncaj nraim rau cov kab proteins (nyob ib ncig ntawm 1.4g SDS / 1g protein), txhais tau hais tias tus nqi ntawm cov protein tam sim no kwv yees li proportional rau nws qhov hnyav molecular.
SDS muab cov protein tsis zoo li cas?
SDS yog cov tshuaj ntxuav tes uas muaj cov saw ntev aliphatic thiab pab pawg sulfate. Cov tshuaj ntxuav tes no cuam tshuam nrog cov proteins uas tsis muaj npe tsim kom muaj qhov tsis zoo them nyiaj ntau (tus nqi tsis zoo tshwm sim los ntawm SO 4 2 − pab pawg ntawm SDS). Cov proteins yog thawj zaug denatured los ntawm tshav kub thiab tom qab ntawd SDS tau ntxiv rau qhov loj tshaj.
