Txog qhov tshwm sim kawg yog hloov pauv hauv lub sijhawm khaws cia uas tau tshwm sim los ntawm kev nce siab rov qab hauv kab. Kev nce siab rov qab tuaj yeem qhia tau tias muaj kab mob sib kis, tab sis txawm tias qhov txhaws frit tuaj yeem cuam tshuam lub sijhawm khaws cia.
Dab tsi ua rau lub sijhawm tuav cia?
Ib qho ntawm feem ntau ua rau hloov pauv hauv lub sijhawm khaws cia hauv qhov hloov pauv-theem LC sib cais yog ib qho kev hloov me me hauv cov concentration ntawm cov kuab tshuaj organic, feem ntau yog methanol lossis acetonitrileQhov no tuaj yeem tshwm sim los ntawm qhov yuam kev me me hauv kev tsim los yog kev hloov pauv ntawm lub xov tooj ntawm tes-theem muaj pes tsawg leeg yog tias ib qho hnyav evapo tus nqi dhau sijhawm.
Yuav ua li cas nce lub sijhawm khaws cia?
Yog tias qhov polarity ntawm qhov chaw nyob ruaj khov thiab qhov sib xyaw ua ke zoo ib yam, lub sijhawm khaws cia nce ntxiv vim tias qhov sib xyaw ua ke muaj zog dua nrog rau theem nyob ruaj khov. Yog li ntawd, cov tebchaw polar muaj lub sijhawm khaws cia ntev ntawm cov theem ntawm cov polar nyob ruaj khov thiab lub sijhawm tuav luv dua ntawm cov kab uas tsis yog-polar siv tib qhov kub.
Dab tsi cuam tshuam rau lub sijhawm khaws cia?
Lub sijhawm khaws cia yog nyob ntawm ntau yam: kev soj ntsuam cov xwm txheej, hom kab, kab kem qhov ntev, degradation ntawm kab, muaj cov ntsiab lus nquag xws li kev sib kis. thiab lwm yam. Yog hais tias xam tus piv txwv paub, tag nrho cov peaks tshwm nyob rau lub sij hawm luv dua thaum koj txiav tawm ib feem ntawm kab.
Dab tsi cuam tshuam rau lub sijhawm khaws cia hauv chromatography?
Ib qhov kev hloov pauv ntawm qhov kub thiab txias feem ntau ua rau lub sijhawm khaws cia ntawm tag nrho cov ncov. Ib qho kev hloov pauv ntawm qhov kub thiab txias, lub sijhawm tuav thawj zaug, lossis tus nqi nce tuaj yeem cuam tshuam tag nrho cov peaks. Lub sij hawm khaws cia nce ntxiv nrog qhov ntsuas kub qis dua, lub sijhawm tuav tau ntev dua, lossis tus nqi qis dua.
