Kev siv ntau tshaj plaws yog rau kev tsim cov Ntshav Agar PhajDehydrated culture media (Columbia Agar) yog ntxiv rau cov dej deionised thiab tom qab ntawd sterilized ntawm 121 ° C. Tom qab ntawd cov molten agar yog txias rau 42 ° C, cov ntshav Nees tshiab los yog yaj cov ntshav yog ntxiv ntawm 5% lossis 7% concentration ua ntej muab nchuav rau hauv petridishes.
Defibrinated ntshav txhais li cas?
tag nrho cov ntshav los ntawm cov fibrin tau raug tshem tawm. Tsis txhob khaus.
Yuav ua li cas ntxuav yaj cov ntshav?
Sterilize by autoclaving at 121°C for 15 minutes. Hloov pauv li no npaj cov ntshav agar puag mus rau 50 ° C dej da dej. Thaum lub hauv paus agar txias rau 50 ° C, ntxiv cov ntshav yaj uas tsis muaj menyuam aseptically thiab sib tov zoo maj mam. Zam kev tsim cov npuas.
Vim li cas thiaj siv cov ntshav Defibrinated?
Ntshav yuav tsum yog defibrinated thaum sau los yog sau rau hauv hnab uas muaj cov tshuaj tiv thaiv coagulant los tiv thaiv kev tsim cov ntshav.
Koj siv Defibrinated plasma li cas?
Plasma tuaj yeem hloov pauv mus rau ntshav los ntawmtxoj kev ntawm defibrination. Coagulation yam tam sim no nyob rau hauv plasma tuaj yeem qhib kom tsim fibrin, nrog rau kev sib ntxiv ntawm calcium chloride thiab thrombin (1). Thrombin cleaves fibrinogen los tsim fibrin monomers, uas polymerize, tsim kom muaj ib qho chaw ruaj khov.
