Spheroids tau tsim los siv paub zoo hanging txoj kevCov qes tawg tau raug tshem tawm hauv kev loj hlob tawm ntawm qhov concentration ntawm 5,000 hlwb / 20 μl. Cov tee ntawm cov tshuaj npaj ntawm tes (20 μl / tee) tau muab tso rau ntawm lub hau ntawm 60-mm daim ntaub kab lis kev cai phaj thiab incubated rau 2 hnub los tsim spheroids.
Spheroids tsim li cas?
Spheroids tuaj yeem loj hlob nrog ob peb txoj kev sib txawv. Ib txoj hauv kev yog siv cov phaj qis ntawm tes, feem ntau yog 96 lub phaj zoo, los tsim cov kab mob spheroid ntau, qhov sib xyaw ua ke hauv qab ntawm cov phiaj hauv qab.
cell spheroids yog dab tsi?
Spheroids, peb-dimensional (3D) kab lis kev cai ntawm tes uas npaj lawv tus kheej thaum loj hlob mus rau hauv cov qauv zoo li tus kheej, tau txais lawv lub npe nyob rau xyoo 1970, thaum cov kws tshawb fawb pom tias lub ntsws hamster. cell loj hlob nyob rau hauv ncua kev kawm ntawv lawv tus kheej nyob rau hauv ib tug yuav luag zoo meej spherical daim ntawv.
Koj ntsuas spheroids li cas?
Analogously, spheroid ntim tuaj yeem siv los ntsuas qhov ua tau zoo rau hauv vitro mob cancer kev tshawb fawb tshuaj. Spheroid ntim ( V=0.5NtevDav 2 ) yog txiav txim siab raws li qhov loj thiab me axial ntev (feem ntau hu ua ntev thiab dav.) ntawm spheroids6, 7
Puas muaj pes tsawg lub hlwb nyob hauv spheroid?
fig. 10. Khoos phis tawj simulation ntawm cov ciaj sia taus ntawm spheroids pib tsim los ntawm 5 × 10 4 hlwb thiab irradiated raws li sib txawv fractionation schemes.
