Cov txheej txheem:
- cell spheroids yog dab tsi?
- Vim li cas cov qog nqaij hlav cancer tsim spheroids?
- Dab tsi yog qhov txawv ntawm spheroids thiab Organoids?
- Kab hlwb li cas?
Video: Spheroids ua li cas?
2024 Tus sau: Fiona Howard | [email protected]. Kawg hloov kho: 2024-01-10 06:38
Spheroids tuaj yeem loj hlob nrog ob peb txoj kev sib txawv. Ib txoj hauv kev yog siv cov phaj qis ntawm tes, feem ntau yog 96 lub phaj zoo, los tsim cov kab lis kev cai spheroid ntau, qhov sib xyaw ua ke hauv qab ntawm cov phiaj ntawm tes.
cell spheroids yog dab tsi?
Spheroids, peb-dimensional (3D) kab lis kev cai ntawm tes uas npaj lawv tus kheej thaum loj hlob mus rau hauv cov qauv zoo li tus kheej, tau txais lawv lub npe nyob rau xyoo 1970, thaum cov kws tshawb fawb pom tias lub ntsws hamster. cells loj hlob nyob rau hauv ncua kev kawm ntawv lawv tus kheej nyob rau hauv ib tug yuav luag zoo meej spherical daim ntawv.
Vim li cas cov qog nqaij hlav cancer tsim spheroids?
Kev tsim ntawm ntau lub cellular spheroidal cell aggregates, los yog spheroids, yog ib qho kev pom zoo ntawm cov kab mob qog noj ntshav (CSCs) thiab qog-initiating hlwb (TICs) uas muaj peev xwm rau kev rov ua dua tshiab, kev loj hlob ntawm tus kheej. thiab tsim cov kab mob qis qis los txhawb cov qog loj hlob [1].
Dab tsi yog qhov txawv ntawm spheroids thiab Organoids?
Organoids yog pawg ntawm cov kab mob tshwj xeeb, xws li hauv plab, siab, lossis zais zis. … Spheroids yog ib pawg ntawm cov hlwb uas muaj ntau yam, xws li los ntawm cov qog nqaij hlav, lub cev embryoid, hepatocytes, paj hlwb, lossis cov qog mammary.
Kab hlwb li cas?
Cell kab lis kev cai hais txog tshem tawm cov hlwb ntawm tus tsiaj lossis cov nroj tsuag thiab lawv txoj kev loj hlob tom qab hauv qhov chaw tsim khoom zoo nkauj… Thaum lub sijhawm no, cov hlwb yuav tsum tau subcultured (ie, hla dhau) los ntawm kev xa lawv mus rau lub nkoj tshiab nrog kev loj hlob nruab nrab kom muaj chaw ntxiv rau kev loj hlob ntxiv.
Pom zoo:
Yuav ua li cas loj npaum li cas ntawm youtube thumbnail?
Image loj & daws teeb meem Muaj daws teeb meem ntawm 1280x720 (nrog qhov dav yam tsawg kawg nkaus ntawm 640 pixels). Muab tso rau hauv cov duab hom xws li JPG, GIF, lossis PNG. Nyob rau hauv qhov txwv 2MB. Sim siv 16: 9 nam piv raws li nws yog qhov siv tshaj plaws hauv YouTube players thiab saib ua ntej .
Txhais li cas rau cov protein kom denatured thiab yuav ua li cas lawv thiaj ua tau zoo?
Denaturation, hauv biology, txheej txheem hloov kho cov qauv molecular ntawm cov protein… Cov proteins uas tau hloov pauv muaj qhov xoob, ntau dua random qauv; feem ntau yog insoluble. Denaturation tuaj yeem coj los ntawm ntau txoj hauv kev-xws li, los ntawm cua sov, los ntawm kev kho nrog alkali, kua qaub, urea, los yog detergents, thiab los ntawm kev tshee tshee .
Yuav ua li cas tsis ncaj ncees txhais li cas?
Kev Ua Phem Tsis Txaus Siab txhais tau tias txhob txwm tsis quav ntsej txog kev ua tau zoo thiab ua tib zoo saib xyuas cov qauv kev ua tau zoo lossis kev coj ua raws li Daim Ntawv Cog Lus nrog kev paub tias yuav ua rau muaj kev raug mob rau ib tus neeg lossis tus neeg lossis poob lossis puas khoom .
Yuav ua li cas thiaj ua tau spheroids?
Spheroids tau tsim los siv paub zoo hanging txoj kevCov qes tawg tau raug tshem tawm hauv kev loj hlob tawm ntawm qhov concentration ntawm 5,000 hlwb / 20 μl. Cov tee ntawm cov tshuaj npaj ntawm tes (20 μl / tee) tau muab tso rau ntawm lub hau ntawm 60-mm daim ntaub kab lis kev cai phaj thiab incubated rau 2 hnub los tsim spheroids .
Yuav sau li cas vim li cas thiab yog vim li cas?
Kev txhais ntawm yog vim li cas thiab (tus) yog li ntawd: yog vim li cas rau ib yam Nws piav qhia vim li cas thiab yog li ntawd ntawm tus nqi nce . Yuav ua li cas thiab yog vim li cas? Txhua qhov laj thawj thiab qhov laj thawj, ib yam li hauv Nws tau mus rau qhov laj thawj thiab yog li ntawm lub koom haum kev saws me nyuam txoj cai thiab cov txheej txheem.
