Raws li tus nqi ntawm cov xim kua dilution raws li cov piv txwv hauv qab no: Ntxiv 1 ml ntawm 1% toluidine xiav stain stock tov(Reagent A) mus rau 9 ml ntawm diluent (Reagent B), thiab sib tov zoo kom tau txais kev ua haujlwm ntawm 0.1% toluidine xiav stain. Toluidine xiav stain ua hauj lwm kua yuav tsum tau siv tam sim.
Dab tsi yog toluidine xiav stain hauv pob txha?
Ua cationic dye, toluidine xiav staining visualizes proteoglycans hauv cov ntaub so ntswgvim nws cov affinity siab rau pawg sulfate hauv proteoglycans. … Tsim: Articular pob txha mos thiab pellets ntawm chondrocytes los yog pob txha pob txha stromal hlwb raug soj ntsuam nrog tus txheej txheem staining rau toluidine xiav.
Yuav ua li cas thiaj ua rau ntsuab stain sai?
- Deparaffinize slides thiab rehydrate rau deionized dej. Stain nrog 0.04% Toluidine Blue Solution rau 10 min.
- Yaug maj mam nrog 3 qhov kev hloov pauv ntawm cov dej deionized (30 sec txhua) Txau nrog 0.02% Fast Green tov rau 3 min.
- Yaug maj mam nrog 2 kev hloov pauv ntawm dej deionized (30 sec txhua)
Koj siv toluidine xiav dye li cas?
1. Txhaum T-Blue swab kov, instilling dye rau hauv lub swab txuas. 2. Siv cov tshuaj toluidine xiav rau thaj chaw ntawm qhov chaw mos sab nraud qhov chaw raug mob tau raug sau tseg ua ntej kev tso cov speculumhauv tus poj niam tus neeg mob.
Puas Toluidine Blue puas tsim kev puas tsuaj?
Nws yog ib qho qhov muag khaus Nws toxicity tsis tau txheeb xyuas tab sis tau ua pov thawj tias muaj tshuaj lom heev rau cov tsiaj txhu thaum txhaj tshuaj intravenously. Lwm cov dyes hauv pab pawg no paub tias muaj tshuaj lom heev, xws li. methylene xiav. … Yog hais tias khaus tshwm sim mus nrhiav kev kho mob tam sim ntawd.
