Cia lawv txias! Cov txheej txheem ntawm kev ua cov hlwb muaj peev xwm yog qhov nyuaj vim qhov xav tau ntawm cov hlwb kom nyob txias. Qhov no yog ib qho tseem ceeb heev vim hais tias cov hlwb yog rhiab heev thiab tsis yooj yim thaum lawv tab tom ua kom muaj peev xwmUa kom qhov kub thiab txias yuav pab kom tsis txhob muaj cell tuag thaum ua haujlwm.
Vim li cas cov kev cuam tshuam khaws cia rau hauv dej khov thaum lub sijhawm sim hloov pauv?
Cov plasmid-cell sib tov ces yog luv luv rau 45-50 ° C, tso cai rau DNA nkag mus rau hauv lub cell los ntawm cov membrane cuam tshuam. Qhov sib xyaw ua kom sov yog muab tso rau hauv dej khov khaws cov plasmids hauv cov kab mob… Yog tias cov tshuaj plasmid DNA muaj ntsev ntau dhau rau hauv nws, arcing tuaj yeem tshwm sim, cuam tshuam qhov kev hloov pauv.
Vim li cas koj tso cov hlab rau dej khov?
Vim li cas koj xav tias peb muab cov hlab rau dej khov? Yuav kom DNA nkag mus rau hauv cov kab mob, peb yuav tsum tso qhov hauv lawv nrog cov tshuaj calcium chloride (CaCl2). … Cov qhov poked tso cai rau DNA nyob rau hauv cov kab mob to. Yog tias peb tsis muab lawv tso rau hauv dej khov, lawv yuav 'los ntshav' kom tuag.
Lub luag haujlwm ntawm cov dej khov txias calcium chloride yog dab tsi hauv kev npaj cov hlwb muaj peev xwm?
Cov txheej txheem ntawm calcium chloride hloov pauv kub-poob siab txhawb cov kab mob hlwb kom nce DNA los ntawm ib puag ncig ib puag ncig. … Cov dej khov-txias CaCl2 daws pab kom khi DNA rau saum npoo ntawm lub xovtooj, uas tom qab ntawd nkag mus rau hauv lub xovtooj tom qab lub sijhawm luv luv ntawm kev kub ntxhov (3).
Koj khaws cov hlwb li cas?
Cov hlwb muaj peev xwm yuav tsum tau khaws cia ntawm - 80 ° C. Cia ntawm -20 ° C yuav ua rau txo qis hauv kev hloov pauv kev ua tau zoo (TE). Thaum kuaj ntawm NEB 5-alpha Competent E. coli (NEB C2987H), cov hlwb poob 94.5% ntawm TE tom qab tsuas yog 24 teev cia ntawm -20 ° C.
