Ampholytes yog siv los tsim pH gradient nyob rau hauv lub capillary, thiab cov proteins kom sib cais migrate (los yog tsom) los ntawm qhov nruab nrab ampholyte kom txog thaum lawv tsis muaj nqi ntawm lawv cov pI nqi.
Vim li cas ampholytes siv hauv IEF?
Nyob rau hauv IEF, ampholytes mus ncig raws li lawv cov nqi nyob rau hauv lub zog ntawm hluav taws xob teb, nyob rau hauv lub xub ntiag ntawm pH gradient, kom txog rau thaum tus nqi ntawm cov molecule yog xoom (Piv txwv li, isoelectric point, pI). … Txawm tias qhov yooj yim tshaj plaws ampholytes (piv txwv li, amino acids) tuaj yeem tsim pH gradient thiab ua raws li isoelectric buffer.
Vim li cas isoelectric point tseem ceeb?
Lub ntsiab lus isoelectric yog qhov tseem ceeb hauv kev ua kom huv ntawm cov protein vim nws sawv cev rau pH qhov twg solubility feem ntau tsawg kawg. … Cov polymer no muaj cov khoom tshwj xeeb los xyuas kom meej tias cov proteins tsim pH gradient thaum koj siv cov hluav taws xob hla kev daws teeb meem.
Ampholytes ua li cas tsim pH gradient?
Nyob hauv CIEF, qhov sib txawv ntawm pH gradient yog tsim hauv cov hlab ntsha los ntawm kev siv hluav taws xob hla cov cab ampholytesQhov dav ntawm pH gradient nyob ntawm seb cov ampholytes yog xaiv. Ampholytes muaj kev lag luam los npog ob qhov dav thiab nqaim pH ntau yam, raws li pom hauv daim duab 5.2.
Ampholytes chemistry yog dab tsi?
Ampholytes yog cov khoom sib xyaw uas thaum yaj hauv dej(uas yog nws cov tshuaj amphoteric) tuaj yeem ua kua qaub lossis ua lub hauv paus.