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Dab tsi elutes thawj zaug nyob rau hauv reversed theem hplc?

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Dab tsi elutes thawj zaug nyob rau hauv reversed theem hplc?
Dab tsi elutes thawj zaug nyob rau hauv reversed theem hplc?

Video: Dab tsi elutes thawj zaug nyob rau hauv reversed theem hplc?

Video: Dab tsi elutes thawj zaug nyob rau hauv reversed theem hplc?
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Nyob rau theem HPLC qhov kev txiav txim ntawm elution yog qhov sib txawv ntawm qhov kev sib cais ib txwm, nrog ntau dua polar solutes eluting thawj. Kev nce lub polarity ntawm lub sijhawm txawb ua rau lub sijhawm khaws cia ntev dua.

Qhov kev sib txuas twg yuav elute thawj zaug hauv qhov hloov pauv HPLC sib cais?

Rov qab-theem chromatography ntiav cov polar (aqueous) mobile theem. Yog li ntawd, hydrophobic molecules nyob rau hauv lub polar mobile theem yuav adsorb mus rau lub hydrophobic nyob twj ywm theem, thiab hydrophilic molecules nyob rau hauv lub mobile theem yuav dhau los ntawm kab thiab eluted ua ntej.

Cov ntsiab lus dab tsi elute ua ntej hauv HPLC?

Least polar analytes elute ua ntej, ntau polar analytes tau khaws cia ntev dua. Tsawg mus rau nruab nrab polarity cov kuab tshuaj yog siv (hexane, ethyl acetate, methanol).

Dab tsi elutes thawj zaug hauv HPLC ib txwm?

Nyob rau hauv ib txwm-theem chromatography, qhov tsawg tshaj plaws polar compounds elute ua ntej thiab feem ntau polar compounds elute kawg. Cov theem ntawm lub xov tooj ntawm tes muaj cov kuab tshuaj tsis muaj polar xws li hexane lossis heptane tov nrog me ntsis ntxiv polar hnyav xws li isopropanol, ethyl acetate lossis chloroform.

Qhov twg yuav elute ua ntej?

Ib tug tsis muaj zog polar hnyav yuav zoo li elute qhov tsawg polar molecules ua ntej. Yog li, hexanetej zaum yuav yog thawj tus neeg tau txais txiaj ntsig, vim tias alkanes yog qhov qis dua polar dua alkenes.

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