Lub sij hawm thim rov qab-theem piav qhia txog hom chromatography uas yog tsuas yog qhov sib txawv ntawm theem ib txwm, uas yog siv lub polar mobile theem thiab tsis-polar [hydrophobic] nyob ruaj khov theem. … A C18-bonded silica [tej zaum hu ua ODS] yog hom nrov tshaj plaws ntawm kev hloov pauv-theem HPLC packing.
Yuav ua li cas nyob rau theem rov qab HPLC?
Rov qab-theem chromatography ntiav cov polar (aqueous) mobile theem. Yog li ntawd, hydrophobic molecules nyob rau hauv lub polar mobile theem yuav adsorb mus rau lub hydrophobic nyob twj ywm theem, thiab hydrophilic molecules nyob rau hauv lub mobile theem yuav dhau los ntawm kab thiab eluted ua ntej.
Dab tsi yog qhov chaw nres tsheb thiab lub xov tooj ntawm tes hauv qhov hloov pauv-theem HPLC?
Nyob rau hauv reversed-theem chromatography, uas yog ntau hom HPLC, theem nyob ruaj ruaj yog nonpolar thiab lub xov tooj ntawm tes yog polar.
Vim li cas thiaj rov qab-theem HPLC nyiam?
Rov qab-theem chromatography muab zoo dua solubility rau polar analytes, siv cov kuab tshuaj tsis muaj tshuaj lom, muaj ib txoj hauv kev tshem tawm cov kab mob thiab cov theem mobile additives, thiab muab cov qauv rov qab raws sijhawm nrog cov kuab tshuaj me me evaporation.
Qhov txawv ntawm HPLC theem ib txwm thiab rov qab yog dab tsi?
Qhov sib txawv tseem ceeb ntawm theem rov qab thiab theem ib txwm HPLC yog qhov rov qab theem HPLC siv lub sijhawm tsis muaj polar nyob ruaj khov thiab theem polar mobilewhereas theem ib txwm HPLC siv lub polar nyob ruaj ruaj theem thiab theem qis polar mobile.
