Cov txheej txheem:
- Yuav ua li cas nyob rau theem rov qab HPLC?
- Dab tsi yog qhov chaw nres tsheb thiab lub xov tooj ntawm tes hauv qhov hloov pauv-theem HPLC?
- Vim li cas thiaj rov qab-theem HPLC nyiam?
- Qhov txawv ntawm HPLC theem ib txwm thiab rov qab yog dab tsi?
Video: Nyob rau hauv reversed theem hplc?
2024 Tus sau: Fiona Howard | [email protected]. Kawg hloov kho: 2024-01-10 06:38
Lub sij hawm thim rov qab-theem piav qhia txog hom chromatography uas yog tsuas yog qhov sib txawv ntawm theem ib txwm, uas yog siv lub polar mobile theem thiab tsis-polar [hydrophobic] nyob ruaj khov theem. … A C18-bonded silica [tej zaum hu ua ODS] yog hom nrov tshaj plaws ntawm kev hloov pauv-theem HPLC packing.
Yuav ua li cas nyob rau theem rov qab HPLC?
Rov qab-theem chromatography ntiav cov polar (aqueous) mobile theem. Yog li ntawd, hydrophobic molecules nyob rau hauv lub polar mobile theem yuav adsorb mus rau lub hydrophobic nyob twj ywm theem, thiab hydrophilic molecules nyob rau hauv lub mobile theem yuav dhau los ntawm kab thiab eluted ua ntej.
Dab tsi yog qhov chaw nres tsheb thiab lub xov tooj ntawm tes hauv qhov hloov pauv-theem HPLC?
Nyob rau hauv reversed-theem chromatography, uas yog ntau hom HPLC, theem nyob ruaj ruaj yog nonpolar thiab lub xov tooj ntawm tes yog polar.
Vim li cas thiaj rov qab-theem HPLC nyiam?
Rov qab-theem chromatography muab zoo dua solubility rau polar analytes, siv cov kuab tshuaj tsis muaj tshuaj lom, muaj ib txoj hauv kev tshem tawm cov kab mob thiab cov theem mobile additives, thiab muab cov qauv rov qab raws sijhawm nrog cov kuab tshuaj me me evaporation.
Qhov txawv ntawm HPLC theem ib txwm thiab rov qab yog dab tsi?
Qhov sib txawv tseem ceeb ntawm theem rov qab thiab theem ib txwm HPLC yog qhov rov qab theem HPLC siv lub sijhawm tsis muaj polar nyob ruaj khov thiab theem polar mobilewhereas theem ib txwm HPLC siv lub polar nyob ruaj ruaj theem thiab theem qis polar mobile.
Pom zoo:
Nyob rau hauv av worm copulatory papillae muaj nyob rau hauv ntu?
Genital papillae yog ob khub ntawm protuberances ntawm ntu 17 thiab 19 ntawm qhov chaw ventralTxhua papilla dais lub khob ntiav zoo li kev nyuaj siab nyob rau sab saum toj uas ua haujlwm zoo li nqus thaum lub sijhawm sib sau. Ntawm ntu 18 muaj ib khub txiv neej qhov chaw mos qhib rau ntawm sab ventral .
Leej twg lub cev nyob rau hauv lub qab nthab nyob rau hauv keeb kwm?
Charlie lub taub hau raug txiav tawm, yog li lub cev ntawm pog– muab tso rau hauv Graham lub tsev pheeb suab los ntawm cov tswv cuab ntawm kev ntseeg - zoo li decapitated, thiab Annie, thaum muaj kev cia siab, pom ntab rau hauv rafters thiab txiav nws lub taub hau tawm (lub sijhawm uas tau muab rau kuv npau suav phem tshaj plaws) .
Nyob rau hauv cov qoob loo cog qoob loo xaiv siv nyob rau hauv?
Clonal xaiv yog txoj kev xaiv ntawm kev ntshaw clones los ntawm cov pejxeem sib xyaw ntawm cov qoob loo vegetatively propagated. Nws yog ib txoj hauv kev txhim kho cov qoob loo vegetatively propagated xws li qab zib, txiv tsawb, qos yaj ywm, citrus, txiv nkhaus taw, thiab lwm yam .
Nyob rau hauv teb rau qhov tuaj txog ntawm acidic chyme nyob rau hauv lub duodenum?
Pab plab hnyuv . theem plab hnyuvtshwm sim hauv duodenum raws li cov lus teb rau cov chyme tuaj txog, thiab nws txo qis kev ua pa ntawm plab los ntawm cov tshuaj hormones thiab lub paj hlwb reflexes . Yuav ua li cas teb rau qhov tuaj txog ntawm acidic chyme hauv duodenum?
Dab tsi elutes thawj zaug nyob rau hauv reversed theem hplc?
Nyob rau theem HPLC qhov kev txiav txim ntawm elution yog qhov sib txawv ntawm qhov kev sib cais ib txwm, nrog ntau dua polar solutes eluting thawj. Kev nce lub polarity ntawm lub sijhawm txawb ua rau lub sijhawm khaws cia ntev dua . Qhov kev sib txuas twg yuav elute thawj zaug hauv qhov hloov pauv HPLC sib cais?
