Ntau cov tshuaj tiv thaiv muaj KD qhov tseem ceeb hauv micromolar qis (10- 6) to nanomolar (10 -7 to 10- 9) ntau. High affinity antibodies feem ntau suav hais tias yog nyob rau hauv qis nanomolar ntau yam (10 - 9) nrog cov tshuaj tiv thaiv kab mob siab heev nyob hauv picomolar (10 -12) ntau yam.
Dab tsi yog qhov tsawg nanomolar potency?
Los ntawm cov qauv kev cai med-chem, ib tus lej nanomolar=zoo, ob tus lej nanomolar=tsis phem, triple-digit nanomolar lossis qis micromolar= pib taw tes ua kom zoo dua, siab micromolar=tsis quav ntsej, thiab millimolar=tuaj yeem ua tau zoo dua nrog cov khoom tawm hauv qab ntawm koj nkawm khau.
Kd qis txhais li cas?
Qhov tseem ceeb tshaj plaws uas yuav tsum nco ntsoov txog Kd yog qhov kev sib raug zoo siab dua, Kd qis dua. … Yog li Kd siab dua txhais tau hais tias thaum koj mus ua ib qho kev suav pej xeem molecular, muaj ntau cov molecules unbound, whereas ib tug qis Kd txhais tau tias uas koj pom ntau khi molecules.
Dab tsi yog qhov sib txawv tsis tu ncua?
Qhov me dua qhov kev sib cais tsis tu ncua, ntau nruj ntawm ligand yog, lossis siab dua qhov kev sib raug zoo ntawm ligand thiab protein. Piv txwv li, ib lub ligand nrog ib tug nanomolar (nM) dissociation tas li khi ntau nruj rau ib qho protein ntau dua li ligand nrog micromolar (μM) dissociation tas li.
Dab tsi yog suav tias yog kev sib cais siab tas li?
Ka thiab pK
Tus logarithmic qhov tsis tu ncua (pKa) yog sib npaug - log 10 (Ka). Qhov loj dua tus nqi ntawm pKa, qhov me dua qhov kev sib cais. Cov kua qaub tsis muaj zog muaj pKa tus nqi hauv thaj tsam ntawm -2 txog 12 hauv dej. Cov kua qaub uas muaj pKa tus nqi tsawg dua li ntawm -2 tau hais tias yog cov acids muaj zog.
