Tshwj xeeb, papain cleaves immunoglobulin G (IgG) molecules ze rau thaj tsam pob khawm ntawm cov tshuaj tiv thaiv , ua rau peb zoo sib xws, ~ 50 kDa, tawg; ib qho Fc domain Fc domain Lub fragment crystallizable cheeb tsam (Fc cheeb tsam) yog thaj tsam tus Tsov tus tw ntawm ib qho tshuaj tiv thaivuas cuam tshuam nrog cov cell nto receptors hu ua Fc receptors thiab qee cov proteins ntawm cov kab ke ntxiv. Cov cuab yeej no tso cai rau cov tshuaj tiv thaiv kab mob ua kom lub cev tiv thaiv kab mob. … Cov cheeb tsam Fc ntawm IgGs dais qhov chaw txuag N-glycosylation heev. https://en.wikipedia.org › wiki › Fragment_crystallizable_region
Fragment crystallizable cheeb tsam - Wikipedia
thiab ob lub tebchaws Fab Kis. Qhov no papain-digested antibody yog li tsis muaj peev xwm los txhawb agglutination, nag lossis daus, opsonization lossis lysis.
papain tshem cov tshuaj tiv thaiv qhov twg?
Papain cleaves cov tshuaj tiv thaiv rau hauv ob lub Fab tawg, uas paub txog cov antigen tshwj xeeb nrog lawv thaj chaw sib txawv, thiab ib qho Fc fragment. Nws cleaves saum lub pob khawm uas muaj cov disulfide bonds uas koom nrog cov chains hnyav, tab sis hauv qab qhov chaw ntawm cov disulfide daim ntawv cog lus ntawm lub teeb sawb thiab cov saw hnyav.
Thaum IgG yog cleaved los ntawm papain uas fragments tshwm?
Papain cleaves immunoglobulin G molecules nyob rau hauv lub pob khawm vim li cas uas tshwm sim nyob rau hauv tiam ntawm peb ~ 50kDa fragments; ob Fab domains thiab Fc domain. Papain-digested antibody tsis tuaj yeem txhawb nqa agglutination, nag lossis daus, opsonization, thiab lysis.
Yuav ua li cas thaum IgG cuam tshuam nrog papain?
Thaum IgG molecules raug tsim nrog papain nyob rau hauv lub xub ntiag ntawm ib tug txo tus neeg sawv cev, ib los yog ntau tshaj peptide daim ntawv cog lus nyob rau hauv lub pob khawm yog sib cais, ua peb feem ntawm qhov loj me: ob Fab fragment thiab ib Fc. fragment (1).
pepsin cleave IgG qhov twg?
Pepsin cleaves qis dua thaj tsam pob khawm ntawm IgG thiab tsim cov F(ab 0)2 fragment nyob rau hauv uas ob lub Fab tseem ceeb sib txuas los ntawm pob khawm.
