Agarose gel electrophoresis feem ntau yog siv rau cais DNA tawg raws li kev txwvendonuclease digestion lossis PCR amplification. Fragments raug kuaj pom los ntawm staining cov gel nrog intercalating dye, ethidium bromide, ua raws li kev pom / kev yees duab nyob rau hauv lub teeb ultraviolet.
YKoj siv agarose gel li cas hauv electrophoresis?
1. Kev npaj ntawm Gel
- Ntsuas qhov tsim nyog ntawm agarose rau hauv lub hwj Erlenmeyer. Agarose gels tau npaj siv w / v feem pua. …
- Ntxiv cov tsis ua haujlwm rau lub khob uas muaj agarose. Swirl mus mix. …
- Melt agarose / tsis sib tov. …
- Ntxiv ethidium bromide (EtBr) rau qhov concentration ntawm 0.5 μg / ml.
YKoj siv gel electrophoresis li cas?
cov ntsiab lus tseem ceeb:
- Gel electrophoresis yog ib qho txheej txheem siv los cais DNA tawg raws li lawv qhov loj me.
- DNA cov qauv raug thauj mus rau hauv qhov dej (indentations) ntawm ib kawg ntawm ib lub gel, thiab cov hluav taws xob tam sim no tau siv los rub lawv los ntawm cov gel.
- DNA tawg yog qhov tsis zoo, yog li lawv txav mus rau qhov zoo electrode.
Lub luag haujlwm tseem ceeb ntawm gel electrophoresis yog dab tsi?
Gel electrophoresis yog ib txoj hauv kev kuaj siv los cais cov sib xyaw ntawm DNA, RNA, lossis cov proteins raws li qhov loj me me.
electrophoresis siv rau dab tsi?
Electrophoresis yog cov txheej txheem kuaj siv cais DNA, RNA, lossis cov protein molecules raws li lawv qhov loj me thiab cov nqi hluav taws xob. Ib qho hluav taws xob tam sim no yog siv los txav cov molecules kom sib cais los ntawm gel.
