Cov ntaub ntawv inhibitor-bound complex feem ntau nyob rau hauv cov ntsiab lus ntawm cov substrate siab thiab ES-I complex tsis tuaj yeem tso cov khoom thaum lub inhibitor yog khi, yog li ua rau txo Vmax. … Yog li, paradoxically, uncompetitive inhibition ob leeg txo Vmax thiab ua rau kom ib tug enzyme affinity rau nws substrate.
Yuav ua li cas tsis sib tw inhibitor cuam tshuam rau Vmax?
Thaum tus tsis sib tw inhibitor ntxiv Vmax hloov, thaum Km tseem tsis hloov. Raws li Lineweaver-Burk zaj duab xis lub Vmax yog txo thaum lub sij hawm ntxiv ntawm ib qho kev sib tw inhibitor, uas yog qhia nyob rau hauv cov zajlus los ntawm ib tug kev hloov nyob rau hauv ob qho tib si txoj kab nqes thiab y-intercept thaum ib tug tsis sib tw inhibitor ntxiv.
Vim li cas Vmax txo qis hauv kev txwv tsis pub sib tw?
Rau qhov sib tw inhibitor, Vmax yog tib yam li rau cov enzyme ib txwm, tab sis Km loj dua. Rau cov noncompetitive inhibitor, Vmax yog qis dua rau cov enzyme ib txwm, tab sis Km yog tib yam. … Cov substrate ntxiv ua rau cov substrate molecules ntau txaus kom tsis tu ncua "sib ntaus" cov inhibitor molecules rau lub enzyme.
Vim li cas Km thiab Vmax yuav txo qis thaum tsis muaj kev sib tw inhibitor ntxiv?
Tsis sib tw inhibitors khi tsuas yog rau cov enzyme-substrate complex, tsis yog rau cov enzyme dawb, thiab lawv txo ob qho tib si kcat thiab Km (qhov txo qis hauv Km stems los ntawm qhov tseeb tias lawv lub xub ntiag rub lub cev tawm mus. los ntawm dawb enzyme mus rau enzyme-substrate complex).
Vmax txo qis txhais li cas?
A qis Vmax txhais tau hais tias lub enzyme ua haujlwm hauv qhov zoo tshaj plaws.
