Tsis sib tw inhibitors khi tsuas yog rau cov enzyme-substrate complex, tsis yog rau cov enzyme dawb, thiab lawv txo qis kcat thiab Km (qhov txo qis hauv Km stems los ntawm qhov tseeb tias lawv lub xub ntiag rub lub cev tawm mus. los ntawm dawb enzyme mus rau enzyme-substrate complex).
Vim li cas Vmax txo qis hauv kev txwv tsis pub muaj kev sib tw?
Cov inhibitors tsis sib xws tuaj yeem khi rau ES complex. Yog li ntawd, cov inhibitors txo Km vim muaj kev sib khi ua tau zoo thiab txo Vmax vim tias lawv cuam tshuam nrog substrate khi thiab hamper catalysis hauv ES complex.
Vim li cas Km txo qis hauv kev txwv tsis pub sib tw Reddit?
Km zoo li txo qis vim tus inhibitor khi cov ES complex, ua rau nws zoo li cov enzyme muaj ntau dua affinity rau lub substrate dua nws ua tau. Vmax kuj txo qis vim tias tus nqi ntawm cov tshuaj tiv thaiv yog inhibited.
Vim li cas ho tsis sib tw inhibitors tsis cuam tshuam Km?
Thaum tus tsis sib tw inhibitor ntxiv Vmax hloov, thaum Km tseem tsis hloov. … Hauv kev txwv tsis pub muaj kev sib tw, tus inhibitor khi rau ib qho chaw allosteric thiab tiv thaiv cov enzyme-substrate complex los ntawm kev ua cov tshuaj tiv thaivQhov no tsis cuam tshuam rau Km (affinity) ntawm cov enzyme (rau lub substrate).
Yuav ua li cas thiaj tsis sib tw inhibitor ua rau ib qho enzyme?
Tsis sib tw inhibitors khi rau enzyme-substrate complex nkaus xwb, tsis yog rau cov enzyme dawb. Lawv distort qhov chaw nquag los tiv thaiv cov enzyme los ntawm kev ua haujlwm catalytically yam tsis tau thaiv kev khi ntawm cov substrateQhov no tsis tuaj yeem tshwm sim nrog ib qho enzyme uas tsuas yog ua ntawm ib lub substrate ib zaug xwb.
