Phosphate-buffered saline (abbreviated PBS) yog ib qho kev daws teeb meem feem ntau siv hauv kev tshawb fawb lom neeg. … Lub buffer pab tswj pH tsis tu ncua. Lub osmolarity thiab ion concentrations ntawm cov kev daws teeb meem phim cov tib neeg lub cev (isotonic).
phosphate buffer siv rau dab tsi?
Phosphate buffers tau siv dav vim tias lawv pab tswj qib pH tas li hauv ib puag ncig tshwj xeeb. Feem ntau hais lus, cov kws tshawb fawb feem ntau sim ua kom muaj pH ntawm 7.4 ntau li ntau tau vim tias cov khoom zoo sib xws ntawm tib neeg lub cev.
PBS lub homphiaj yog dabtsi?
PBS (phosphate buffered saline) yog cov kua ntsev sib npaug siv rau ntau hom kab lis kev cai ntawm tes, xws li ntxuav cov hlwb ua ntej dissociation, thauj cov hlwb lossis cov ntaub so ntswg, diluting hlwb rau suav, thiab npaj cov tshuaj reagents.
Vim li cas koj thiaj siv PBS?
PBS muaj ntau yam siv vim nws isotonic thiab tsis muaj tshuaj lom rau cov hlwb… PBS tuaj yeem siv los ua cov txheej txheem kom qhuav biomolecules, raws li cov dej molecules nyob rau hauv nws yuav tsim nyob ib ncig ntawm cov khoom (cov protein, piv txwv li) yuav 'qhuav' thiab immobilized rau ib tug khoom nto.
Vim li cas thiaj tseem ceeb siv PBS los ntxuav hlwb?
PBS muaj ntau yam siv vim nws is isotonic thiab tsis muaj tshuaj lom rau cov hlwb feem ntau… Ib yam li ntawd, nws muab cov hlwb nrog dej thiab qee yam inorganic ions tseem ceeb rau cov cell metabolism.
