Ib tug tsis muaj zog polar hnyav yuav zoo li elute qhov tsawg polar molecules ua ntej. Yog li, hexane yuav tej zaum yuav yog thawj tus yuav tsum tau eluted, vim alkanes yog HLUB tsawg dua polar dua alkenes.
Dab tsi yuav elute thawj zaug hauv chromatography?
Yog li polar molecules tau khaws cia rau hauv kem, koj qhov kev hloov pauv ntawm cov molecules yuav mus ntawm tsis yog-polar mus rau polar. Rau reversed-theem chromatography yam yog, zoo, rov qab. Koj siv cov theem uas tsis yog-polar nyob ruaj ruaj uas khaws cov tsis-polar tebchaw thiab yog li, koj elute thawj cov polar molecules.
Yam kev elution yog dab tsi?
Qhov kev txiav txim ntawm elution thaum siv polydimethyl siloxane feem ntau ua raws cov ntsiab lus kub ntawm cov kua nplaum, nrog qis boiling solutes eluting ua ntej. Hloov qee cov methyl pawg nrog rau lwm cov khoom siv ntxiv ua rau cov theem nyob ruaj khov polarity thiab muab kev xaiv ntau dua.
Qhov twg yuav elute sai dua?
Tus siab dua qhov feem pua ntawm cov kuab tshuaj polar, cov sib txuas nrawm dua yuav elute.
Yuav ua li cas thiaj yuav cov tebchaw elute los ntawm ib kem hauv kab chromatography?
Hauv kab chromatography feem ntau cov tsis muaj polar elute los ntawm kem ua ntej, thiab feem ntau cov polar compounds elute kawg.
Size exclusion chromatography-2
