Qhov kev sim biuret, tseem hu ua Piotrowski qhov kev sim, yog kev sim tshuaj siv los kuaj pom muaj peptide bonds. Nyob rau hauv lub xub ntiag ntawm peptides, ib tug tooj liab (II) ion tsim mauve-xim sib koom ua ke complexes nyob rau hauv ib tug alkaline tov.
Yuav ua li cas yog txoj kev ntsuas biuret?
Txoj kev biuret yog cov txheej txheem colorimetric tshwj xeeb rau cov protein thiab peptidestooj liab ntsev hauv cov tshuaj alkaline tsim cov xim liab qab nrog cov tshuaj uas muaj ob lossis ntau dua peptide bonds. … Yog li, cov tshuaj tiv thaiv biuret nrog cov protein yog tsim nyog rau kev txiav txim siab ntawm tag nrho cov protein los ntawm spectrophotometric (ntawm 540–560 nm).
Yuav kuaj biuret li cas rau cov protein?
Biuret test rau cov proteins
- Muab ib-ob lub spatula ntawm cov qauv zaub mov rau hauv ib lub raj kuaj los yog 1 cm 3 yog cov qauv yog kua. …
- Ntxiv qhov sib npaug ntawm cov tshuaj potassium hydroxide rau hauv lub raj thiab do.
- Ntxiv ob tee ntawm tooj liab sulfate tov thiab do rau ob feeb.
- Qhia cov xim ntawm cov tshuaj.
Lub hom phiaj ntawm kev kuaj biuret yog dab tsi?
Biuret test yog siv rau kev kuaj xyuas cov tebchaw nrog peptide bonds. Ib qho biuret reagent tuaj yeem siv los kuaj cov qauv aqueous. Qhov xiav reagent no yog tsim los ntawm kev sib txuas ntawm sodium hydroxide thiab tooj liab sulfate daws.
Dab tsi yog tshuaj biuret?
Peb siv Biuret's reagent los kuaj pom muaj cov proteins hauv cov tshuaj. Cov reagent yog daj ntseg xiav thaum ntshiab, tab sis thaum sib xyaw nrog cov proteins, qhov tshwm sim tshwm sim ua rau xim daj ntseg.
