Citrate inhibits phosphofructokinase los ntawm kev txhim kho cov nyhuv inhibitory ntawm ATP. … Fructose 2, 6-bisphosphate activates phosphofructokinase los ntawm kev ua kom nws cov affinity rau fructose 6-phosphate thiab txo qis inhibitory nyhuv ntawm ATP (Daim duab 16.18).
Koj puas xav tias citrate yuav qhib lossis inhibit Phosphofructokinase?
Citrate allosterically inhibits phosphofructokinase 1, tiv thaiv lub voj voog tsis zoo nrog F1, 6-BP. Nce ATP concentrations inhibit glycolysis thaum muab lub zog rau gluconeogenesis.
Vim li cas citrate yog ib qho allosteric inhibitor?
Nyob hauv haiv neeg enzyme, zoo li citrate yog daig nyob rau hauv qhov sib txawv ntawm N- thiab C-terminal qhov chaw ntawm cov protein (12), txij li cov chaw khi ntawm ob qho tib si. ib nrab ua lub luag haujlwm hauv nws cov txiaj ntsig allosteric.
Dab tsi inhibits kev ua ntawm Phosphofructokinase?
ATP yog lub ntuj allosteric inhibitor ntawm PFK, txhawm rau tiv thaiv qhov tsis tsim nyog ntawm ATP los ntawm glycolysis. Txawm li cas los xij, kev hloov pauv hauv Asp(543)Ala tuaj yeem ua rau ATP muaj cov nyhuv inhibitory muaj zog (vim muaj kev sib txuas ntxiv rau PFK's inhibitory allosteric binding site).
Puas citrate khi rau PFK?
Nws feem ntau paub tias citrate tsis cuam tshuam rau cov kab mob PFK1; Txawm li cas los xij, qee qhov inhibition los ntawm citrate tuaj yeem pom hauv PFK1 enzymes los ntawm eukaryotic microbial hom. Nyob rau hauv filamentous fungus Aspergillus niger, enzyme kev ua tau inhibited los ntawm 50% los ntawm koob tshuaj citrate li ntawm 4 mus rau 6 mM [11], [12].
