Qhov kev tshawb pom ntawm kev txwv enzymes pib nrog kev xav. Xyoo 1960, Werner Arber tau pom qhov kev hloov pauv loj hauv cov kab mob bacteriophage DNA tom qab nws nkag mus rau cov kab mob resistant no: Nws tau degraded thiab txiav mus rau hauv daim.
Leej twg nrhiav pom thawj qhov txwv endonuclease?
Nyob rau hauv daim ntawv ntawd, Kathleen Danna thiab Daniel Nathans ntawm Johns Hopkins University (B altimore) tau qhia thawj zaug uas txwv tsis pub enzyme hu ua "endonuclease R," nrhiav tau los ntawm Hamilton Smith thiab Kent Wilcox (2), tuaj yeem siv los tsim cov kab mob tshwj xeeb ntawm tus kab mob simian 40 (SV40) DNA.
Dab tsi yog thawj qhov txwv endonuclease?
Thawj qhov txwv tsis pub muaj nuclease cais tawm los ntawm cov kab mob Haemophilus influenzae. Lub enzyme ( HindII) txiav ntawm ib qhov chaw tshwj xeeb hauv ib ntu ntawm rau lub hauv paus khub raws li hauv qab no.
Leej twg nrhiav kev txwv enzyme hauv xyoo 1972?
Paul Berg, biochemist ntawm Stanford uas yog thawj tus tsim cov DNA molecule recombinant xyoo 1972, tau sau ib tsab ntawv tsis ntev tom qab ntawd, nrog rau kaum lwm tus kws tshawb fawb, mus rau Phau ntawv Journal Science.
Leej twg pom Hind 2?
(Science: enzyme molecular biology) thawj hom II txwv endonuclease txheeb xyuas, los ntawm Hamilton Smith hauv xyoo 1970. cais tawm ntawm haemophilus influenzae, nws cleaves qhov sib lawv liag GTPyPuAC nruab nrab ntawm cov pyrimidine tsis tau teev tseg. purine tsim blunt kawg.
