α-Amanitin muaj qhov txawv txav tsis zoo thiab tshwj xeeb nyiam rau enzyme RNA polymerase II. Thaum noj thiab siv los ntawm daim siab hlwb, nws khi rau RNA polymerase II enzyme, ua rau cytolysis ntawm hepatocytes (lub siab hlwb).
Amanitin inhibit RNA polymerase li cas?
α-Amanitin-Inhibiting RNA Polymerases II thiab III
Amanitin khi nrog cov ntsiab lus siab thiab siab affinity (Ki=3–4 nM) ze ntawm cov catalytic active site ntawm RNAP II. Nws ntxiab ib qho kev sib haum xeeb ntawm cov enzyme uas tiv thaiv nucleotide incorporation thiab translocation ntawm cov ntaub ntawv transcript
Alpha Amanitin cuam tshuam li cas rau kev sau ntawv?
Alpha amanitin inhibits RNA polymerase II (pol II) los ntawm kev thaiv kev pib thiab elongation ntawm kev sau ntawv Nws tau raug pom tias khi lub pol II hauv qab tus choj helix thiab hla lub cleft ntawm Rpb1 thiab Rpb2 (1). Feem ntau ntawm cov seem ntawm pol II uas sib cuam tshuam yog nyob ntawm tus choj helix.
Alpha Amanitin inhibit dab tsi?
α-Amanitin tua cov hlwb los ntawm inhibiting RNA polymerase II (Pol II) thiab kaw cov noob caj noob ces.[1] α-Amanitin yog tsim los ntawm cov protein, ntawm ribosomes, 34 mus rau 35 amino acids nyob rau hauv ntev thiab ces cleaved ntawm tej proline residues los ntawm ib tug enzyme uas muaj nyob rau hauv lub tsev neeg prolyl oligopeptidase (POP) subfamily.
RNA polymerase twg yog qhov nkag siab tshaj plaws rau alpha Amanitin?
RNA polymerase II yog qhov nkag siab tshaj plaws (50% inhibition ntawm 1.0 mug ntawm alpha-amanitin ib ml).
