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Rau ntsuab fluorescent protein?

Cov txheej txheem:

Rau ntsuab fluorescent protein?
Rau ntsuab fluorescent protein?

Video: Rau ntsuab fluorescent protein?

Video: Rau ntsuab fluorescent protein?
Video: cia muab rau niam ntsuab teev tham_ tub huas xyooj 2022-3 2024, Tej zaum
Anonim

Cov protein fluorescent ntsuab yog cov protein uas nthuav tawm cov fluorescence ntsuab ntsuab thaum raug lub teeb xiav rau ultraviolet ntau yam. Daim ntawv lo GFP ib txwm hais txog cov protein ua ntej cais tawm ntawm jellyfish Aequorea victoria thiab qee zaum hu ua avGFP.

Yuav ua li cas activates ntsuab fluorescent protein?

Green fluorescent protein (GFP) yog cov protein nyob hauv jellyfish Aequorea Victoria uas nthuav tawm ntsuab fluorescence thaum raug teeb. … Hauv cov jellyfish, GFP cuam tshuam nrog lwm cov protein, hu ua aequorin, uas tawm lub teeb xiav thaum ntxiv nrog calcium.

Koj ua li cas kuaj cov protein fluorescent ntsuab?

Flow cytometry thiab fluorescent microscopy yog ob lub cuab yeej siv los ntsuas GFP teeb liab; Flow cytometry yog cov txheej txheem ua tau zoo thiab rhiab heev rau kev txheeb xyuas ntau yam fluorescent siv, thaum fluorescent microscopy tuaj yeem pom qhov chaw subcellular thiab qhia txog GFP.

Ntawm ntsuab fluorescent protein tuaj qhov twg?

Green fluorescent protein (GFP) yog cov protein uas tsim los ntawm cov jellyfish Aequorea victoria, uas emits bioluminescence nyob rau thaj tsam ntsuab ntawm qhov pom spectrum. GFP noob tau raug cloned thiab siv hauv molecular biology ua tus cim.

Dab tsi ua rau cov fluorescent ci ntsa iab?

Cov kev daws teeb meem ntawm purified GFP saib daj nyob rau hauv cov teeb pom kev zoo hauv chav, tab sis thaum coj mus rau sab nraum lub hnub ci, lawv ci nrog cov xim ntsuab ci. Cov protein nqus lub teeb ultraviolet los ntawm lub hnub ci, thiab tom qab ntawd tawm nws ua lub teeb ntsuab qis zog.

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