Capillary electrophoresis yog tsev neeg ntawm txoj kev sib cais electrokinetic ua nyob rau hauv submillimeter txoj kab uas hla capillaries thiab hauv micro- thiab nanofluidic channels.
capillary electrophoresis siv rau dab tsi?
Capillary electrophoresis (CE) yog thawj txoj kev siv rau sib cais thiab kuaj xyuas luv tandem rov (STR) alleles hauv cov chaw soj ntsuam DNA thoob ntiaj tebTshooj no tshuaj xyuas cov ntsiab lus dav dav thiab cov khoom ntawm txhaj tshuaj, sib cais, thiab tshawb pom ntawm STR alleles siv CE.
Kev piav qhia yooj yim capillary electrophoresis yog dab tsi?
Capillary electrophoresis yog cov txheej txheem tshuaj ntsuam xyuas uas cais cov ions raws li lawv cov kev txav mus los ntawm electrophoretic nrog kev siv qhov hluav taws xob thov… Cov hom kab nruab nrab tsis cuam tshuam, tsuas yog ions txav nrog lub tshuab hluav taws xob. Yog tias ob lub ions loj tib yam, ib qho uas muaj nqi ntau dua yuav txav nrawm tshaj plaws.
Dab tsi yog capillary electrophoresis hauv DNA?
Capillary electrophoresis (CE) yog lwm txoj rau cov pa slab gel electrophoresis rau kev sib cais ntawm DNA fragments … Qhov ntau ntawm DNA xav tau kev sib cais yog nyob rau hauv ntau nanogram. Ib qho kev daws teeb meem tuaj yeem tau txais ntawm cov khoom tawg mus txog ntau pua tus khub.
Yuav ua li cas tswj tau siv hauv capillary electrophoresis?
Nws koom nrog kev sib cais ntawm peptides los ntawm kev thov rau fused silica capillary (feem ntau 100 cm × 100 μm). Electrophoretic mobility yog tswj los ntawm ib qho hluav taws xob sab nraudthiab kev xaiv tuaj yeem tswj hwm los ntawm ntau yam xws li hnyav pH, lub zog ionic, thiab lwm yam khoom ntxiv.