Rau cov kev daws teeb meem nrog siab viscosity lossis nyiam ua npuas ncaujsiv cov txheej txheem thim rov qab: Cov txheej txheem no feem ntau siv nrog cov pa xa hluav taws xob, thiab tau pom zoo rau cov khoom ntim me me. Rov qab pipetting zam qhov kev pheej hmoo ntawm cov qauv txaws, foaming, los yog npuas tsim.
rov qab pipetting siv rau dab tsi?
Rov qab pipetting yog thev naus laus zis los faib cov kua ntsuas ntsuas los ntawm kev hloov pauv huab cua, uas txo qhov kev pheej hmoo ntawm txaws, ua npuas ncauj lossis npuas. Rov qab pipetting yog qhov tseeb dua hauv kev xa cov kua me me uas muaj cov protein thiab cov tshuaj lom neeg sib piv rau kev xa mus tom ntej.
Vim li cas thim rov qab pipett yog qhov tseeb dua?
Kev siv cov txheej txheem thim rov qab tuaj yeem txhim kho qhov tseeb thaum tso cov kev daws teeb meem, txij li nws aspirates lub ntim uas ntau dua li qhov teeb tsa Cov kua ntau dhau ua raws li cov dej ntim rau txawm tias tawm ntawm cov khoom sib txuas, yog li tswj kev ncaj ncees thiab kev ntseeg siab tom qab ntawm cov ntaub ntawv nrog cov kua hom.
Dab tsi yog qhov txawv ntawm thim rov qab thiab xa mus tom ntej?
Forward pipeting yog txheej txheem txheej txheem rau feem ntau cov kev daws teeb meem aqueous. Rov qab pipetting yog pom zoo rau viscous lossis foaming kua nrog rau cov ntim me me. Lub tshuab nqus tawm yog ntxiv rau qhov xav tau hauv thawj kauj ruam thiab nyob twj ywm hauv lub taub hau kom muab pov tseg.
Yuav ua li cas thiaj tiv thaiv npuas los ntawm pipet?
Tsom rau cov ces kaum: Txhawm rau kom koj tso tag nrho cov kua hauv koj lub popette thiab tsis txhob muaj cua npuas, nqus ntawm 90 degree lub kaum sab xis thiab xa tawm ntawm lub kaum sab xis 45 degree. Tso cov pipettes maj mam: Tom qab tso cov kua hauv koj lub pipette, koj yuav tsum tsis txhob tso lub plunger sai heev.
