Nyob rau hauv thaj chaw ntawm molecular biology, gene qhia profiling yog qhov ntsuas ntawm kev ua haujlwm ntawm ntau txhiab tus noob ib zaug, los tsim cov duab thoob ntiaj teb ntawm kev ua haujlwm ntawm tes. Cov ntaub ntawv no tuaj yeem, piv txwv li, paub qhov txawv ntawm cov hlwb uas nquag faib, lossis qhia tias cov hlwb ua li cas rau kev kho mob tshwj xeeb.
Dab tsi yog qhov profile txhais li cas?
Qhov no yog ib hom kev kawm nrov tshaj plaws, tseem hu ua 'kev nthuav qhia profile'. Nws suav nrog qhov muaj nuj nqis ntawm cov noob qhia ntawm ntau cov noob hauv cov hlwb lossis cov qauv ntaub so ntswg ntawm kev sau npe(RNA) qib.
Dab tsi yog kev tshuaj ntsuam xyuas?
Genome-wide transcription analysis yog ib lub cuab yeej muaj zog rau kev txiav txim siab ua tiav ntawm mRNAs thiab lawv cov txheeb ze qhia qib raws li kev ua haujlwm ntawm kev loj hlob. Txhua qhov kev tshawb fawb luam tawm rau hnub tim ntawm genome-wide transcription hauv S.
Dab tsi yog genome-wide transcriptional profileing?
Kev hloov pauv cov ntaub ntawv nrog DNA microarrays tuaj yeem yog siv los ntsuas cov genome-wide transcriptional teb rau cov molecules me… thiab pab txhawb kev tsom xam ntawm txoj hauv kev downstream ntawm lub hom phiaj.
Koj ua li cas gene qhia profileing?
qPCR yog cov txheej txheem kub-txheej txheem rau kev lees paub qhov sib txawv ntawm cov noob qhia profiles, thiab ua rau:
- Quantition of gene products.
- Microarray validation.
- Pathway analysis.
- Studies of developmental biology.
- Kev tswj hwm zoo thiab kev tshuaj xyuas qhov tseeb.
- siRNA/RNAi kev sim.
- qe-fold luam tus lej kev ntxub ntxaug (down rau ob-fold)
