Phosphate buffered formalin Feem ntau siv formaldehyde-raws li fixative rau niaj hnub histopathology. Qhov tsis tuaj yeem tiv thaiv kev tsim ntawm formalin pigment.
Puas muaj qhov kho zoo tshaj plaws hauv histopathology?
Qhov feem ntau siv fixative hauv histology yog formaldehyde Nws feem ntau yog siv los ua 10% nruab nrab buffered formalin (NBF), uas yog kwv yees. 3.7%–4.0% formaldehyde nyob rau hauv phosphate tsis, pH 7. … Paraformaldehyde kuj nquag siv thiab yuav depolymerise rov qab rau formalin thaum rhuab, kuj ua rau nws ib tug zoo fixative.
Yuav ua li cas yog cov fixative siv nyob rau hauv histopathology?
Formaldehyde (10% nruab nrab buffered formalin) yog nyob deb ntawm cov khoom siv nrov tshaj plaws siv hauv histology txij li nws nkag mus rau cov ntaub so ntswg zoo thiab tsim cov kab sib txuas yam tsis cuam tshuam rau cov qauv ntaub so ntswg antigenicity.
Dab tsi yog qhov kho kom zoo tshaj plaws?
Ib qho tshuaj kho zoo tshaj plaws yuav tsum: Tswj cov ntaub so ntswg thiab cov hlwb kom zoo li lub neej raws li qhov ua tau, tsis muaj qhov ntsws los yog o thiab tsis cuam tshuam los yog ua kom cov cellular constituents. … Ua kom ruaj khov thiab tiv thaiv cov ntaub so ntswg thiab hlwb tiv thaiv kev puas tsuaj ntawm cov txheej txheem tom ntej thiab staining.
Tus nqi zoo tshaj ntawm kev siv tshuaj yog dab tsi?
A fixative rau cov ntaub so ntswg ntawm 20: 1 yog suav hais tias yog qhov qis tshaj qhov pom tau tab sis kuv xav tawm tswv yim rau lub hom phiaj piv ntawm 50:1.
