Cov txheej txheem:
- Yuav ua li cas ua kom bimolecular fluorescence ntxiv ua haujlwm?
- Kev xeem BiFC yog dab tsi?
- Vim li cas BiFC xeem dhau?
- Puas BiFC thim rov qab?
Video: Hauv vivo bimolecular fluorescence ntxiv?
2024 Tus sau: Fiona Howard | [email protected]. Kawg hloov kho: 2024-01-10 06:38
Bimolecular Fluorescence Complementation (BiFC) assay yog ib txoj kev siv los saib pom cov protein-protein cuam tshuam hauv vivo sivcov duab nyob hauv cell lossis cov hlwb tas. … BiFC tau piav qhia thawj zaug los ntawm Hu et al.
Yuav ua li cas ua kom bimolecular fluorescence ntxiv ua haujlwm?
Lub hauv paus ntsiab lus ua haujlwm ntawm BiFC yog ua raws li kev tsim cov fluorescent complex, vim yog kev sib koom ua ke ntawm ob ntu ntawm cov protein fluorescent thaum lawv nyob ze vim muaj cov protein-cov protein sib cuam tshuam hauv cov khoom tawg, piv txwv li., hauv BiFC, fluorophore muab faib ua amino thiab carboxyl terminal kawg.
Kev xeem BiFC yog dab tsi?
bimolecular fluorescence complementation (BiFC) assay ua kom yooj yim thiab ncaj qha pom ntawm cov protein sib cuam tshuam hauv cov hlwb(45). BiFC txoj hauv kev yog ua raws li kev tsim cov fluorescent complex thaum ob lub proteins fused rau cov khoom tsis muaj fluorescent ntawm cov fluorescent protein cuam tshuam nrog ib leeg (Fig.
Vim li cas BiFC xeem dhau?
Lub tswv yim ntawm BiFC yog qhia txog ib nrab ntawm GFP fused rau koj cov proteins uas txaus siab Yog tias POI cuam tshuam, koj tuaj yeem nqa ob qho tib si ua ke kom tau txais GFP ua haujlwm. Yog li yog tias muaj ib nrab ntawm GFP qhia hauv lub xov tooj, kuv tsis xav tsis thoob tias GFP cov tshuaj tiv thaiv tuaj yeem ntes nws.
Puas BiFC thim rov qab?
Qhov tsis tuaj yeem ntawm BiFC complexes tau raug sau tseg zoo (10), thiab nws yuav tshwm sim tias feem ntau, yog tias tsis yog tag nrho, fluorescent protein - raws li BiFC systems yog irreversible (8, 33, 43, 48–51).
Pom zoo:
Puas yog dej yog cov khoom siv txuas ntxiv mus ntxiv?
Piv rau lwm cov peev txheej uas siv los tsim lub zog thiab lub zog, dej suav tias yog rov ua dua tshiab nrog rau cov khoom pov tseg tsawg kawg thaum lub zog tsim khoom . Vim li cas dej thiaj suav tias rov tsim dua tshiab? Dej evaporates ntawm lub ntiaj teb saum npoo thiab nce mus rau hauv qhov chaw uas nws condenses.
Yuav txuas ntxiv lossis txuas ntxiv?
Siv txuas ntxiv thaum koj tab tom hais txog qhov muaj uas yuav txuas ntxiv mus rau yav tom ntej. "Txuas ntxiv" yog kev siv zog ua txhua yam txuas ntxiv mus rau yav tom ntej tab sis tuaj yeem tso tseg txhua lub sijhawm thiab yog vim li cas, "
Ntxiv cov lus txuas ntxiv?
Thiab, ntxiv rau, ntxiv, ntxiv rau, dua li, dua, dhau, kuj, ob qho tib si-thiab, lwm yam, qhov tseem ceeb sib npaug, thawj, thib ob, thiab lwm yam, dua, ntxiv, kawg, kawg, tsis tsuas yog-tab sis kuj, nrog rau, nyob rau hauv qhov chaw thib ob, tom ntej, zoo ib yam, qhov tseeb, qhov tshwm sim, yog li ntawd, tib txoj kev, piv txwv li, piv txwv li, … Cov lus txuas twg qhia ntxiv cov ntaub ntawv?
Qhov txawv ntawm qhov ntxiv thiab qhov ntxiv yog dab tsi?
Addendum yog ib lub npe uas hais txog ib daim ntawv ntxiv rau qhov kawg ntawm ib yam dab tsi. Addenda yog nws plural. … Siv cov ntaub ntawv ntxiv rau hauv daim ntawv qhia ib leeg. Siv addenda hauv ntau daim ntawv . Yuav ua li cas yog ntau tshaj plural rau addendum?
Puas ntxiv cov nplooj ntawv ntxiv?
o Nplooj ntawv tus lej ntawm cov ntawv ntxiv yuav tsum ua raws li nplooj ntawv kawg ntawm cov ntawv thesis lossis cov ntaub ntawv pov thawjQhov no txhais tau hais tias tus lej xov tooj thiab kab lus nrog rau nplooj ntawv sib raug tus lej yuav tsum tau teev tseg.
