Bimolecular Fluorescence Complementation (BiFC) assay yog ib txoj kev siv los saib pom cov protein-protein cuam tshuam hauv vivo sivcov duab nyob hauv cell lossis cov hlwb tas. … BiFC tau piav qhia thawj zaug los ntawm Hu et al.
Yuav ua li cas ua kom bimolecular fluorescence ntxiv ua haujlwm?
Lub hauv paus ntsiab lus ua haujlwm ntawm BiFC yog ua raws li kev tsim cov fluorescent complex, vim yog kev sib koom ua ke ntawm ob ntu ntawm cov protein fluorescent thaum lawv nyob ze vim muaj cov protein-cov protein sib cuam tshuam hauv cov khoom tawg, piv txwv li., hauv BiFC, fluorophore muab faib ua amino thiab carboxyl terminal kawg.
Kev xeem BiFC yog dab tsi?
bimolecular fluorescence complementation (BiFC) assay ua kom yooj yim thiab ncaj qha pom ntawm cov protein sib cuam tshuam hauv cov hlwb(45). BiFC txoj hauv kev yog ua raws li kev tsim cov fluorescent complex thaum ob lub proteins fused rau cov khoom tsis muaj fluorescent ntawm cov fluorescent protein cuam tshuam nrog ib leeg (Fig.
Vim li cas BiFC xeem dhau?
Lub tswv yim ntawm BiFC yog qhia txog ib nrab ntawm GFP fused rau koj cov proteins uas txaus siab Yog tias POI cuam tshuam, koj tuaj yeem nqa ob qho tib si ua ke kom tau txais GFP ua haujlwm. Yog li yog tias muaj ib nrab ntawm GFP qhia hauv lub xov tooj, kuv tsis xav tsis thoob tias GFP cov tshuaj tiv thaiv tuaj yeem ntes nws.
Puas BiFC thim rov qab?
Qhov tsis tuaj yeem ntawm BiFC complexes tau raug sau tseg zoo (10), thiab nws yuav tshwm sim tias feem ntau, yog tias tsis yog tag nrho, fluorescent protein - raws li BiFC systems yog irreversible (8, 33, 43, 48–51).
