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Puas yog centrifugation lyse hlwb?

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Puas yog centrifugation lyse hlwb?
Puas yog centrifugation lyse hlwb?

Video: Puas yog centrifugation lyse hlwb?

Video: Puas yog centrifugation lyse hlwb?
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A ib leeg qis g-force centrifugation kauj ruam ua rau me me cell lysis thiab tiv thaiv kev sib cuag ntawm cov nuclei nrog rau ib puag ncig cytoplasmic. Txoj kev nrawm no qhia tau hais tias muaj kev rov tsim dua tshiab vim yog qhov kev siv ntawm tes me me uas yuav tsum tau ua los ntawm tus neeg tshawb xyuas.

Centrifugation ua li cas rau cov hlwb?

Centrifugation ntawm qhov sib txawv tso cai rau kev sib cais ntawm cov khoom mus rau hauv 'fractions', raws li lawv tau nkag siab npaum li cas (Box 1). Piv txwv li, ntawm qhov qis centrifugation ceev, cov hlwb loj tuaj yeem raug cais tawm ntawm cov hlwb me.

Puas centrifuging tshem tawm cov hlwb tuag?

Kev Sib cais ntawm Cov Tuag thiab Cov Neeg Nyob Los Ntawm Centrifugation

Ib txoj hauv kev yooj yim tshaj plaws ntawm kev tshem tawm cov khib nyiab ntawm tes yog ntom-gradient centrifugation. Qhov ceev-gradient centrifugation harnesses ib lub cuab yeej hu ua lub centrifuge uas tig ib qho sib xyaw heterogenous ntawm kev kub ceev.

Yuav ua li cas thiaj li mob hlwb?

Cov txheej txheem suav nrog khov lub xovtooj ntawm kev ncua hauv qhov qhuavdej khov / ethanol da dej lossis lub tub yees thiab tom qab ntawd muab cov khoom pov tseg hauv chav sov lossis 37 ° C. Cov txheej txheem ntawm lysis no ua rau cov hlwb o thiab thaum kawg tawg raws li cov dej khov nab kuab tsim thaum lub sij hawm khov thiab tom qab ntawd cog lus thaum thawing.

Yuav ua li cas thaum koj centrifuge hlwb nrawm dhau?

Spinning nrawm dhau tuaj yeem ua rau "smear" ntawm cov cell mus rau phab ntsa ntawm lub raj uas koj yuav ploj thaum rov ua duacov hlwb.

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