Cov txheej txheem:
- Kuv tuaj yeem khaws RIPA ntev npaum li cas?
- Puas RIPA tsis tuaj yeem khaws cia ntawm?
- Koj khaws RIPA lysates li cas?
- Yuav ua li cas thaw RIPA buffer?
Video: Puas ripa tsis tuaj yeem khov?
2024 Tus sau: Fiona Howard | [email protected]. Kawg hloov kho: 2024-01-10 06:38
Ib leeg tuaj yeem khov thiab thaw RIPA tsis pub dhau 2-3 zaug yam tsis poob nws lub zog yog tias nws khaws cia rau hauv dej khov thaum nws siv (20-30 feeb). Khaws qhov chaw tsis khov ntawm -80 degree C.
Kuv tuaj yeem khaws RIPA ntev npaum li cas?
1. Yog tias tsis siv yuav txuas ntxiv mus, nws raug pom zoo kom 10x tsis nyob ntawm 4 ° C rau 1-2 lub lis piam. Rau lub sij hawm ntev, tsis yuav tsum tau khaws cia ntawm -20 ° C. Aliquoting ntawm 10x tsis yog pom zoo yog tias yuav tsum tau ua ntau qhov kev sim me me.
Puas RIPA tsis tuaj yeem khaws cia ntawm?
Npaj RIPA tsis pub yuav tsum tau muab tso tseg thiab khaws cia ntawm −20°C. Ntxiv protease thiab/los yog phosphatase inhibitors rau thawed aliquot ua ntej siv tam sim. Ib milliliter ntawm tsis txaus yog txaus rau lyse kwv yees li 5 lab hlwb. …
Koj khaws RIPA lysates li cas?
Siv cov lysates kom sai li sai tau, thiab khaws cia kom ntev li ntev tau. Khaws lysates ntawm -80 ℃ kom ntev li ntev tau. Rau cov lysates uas yuav tsum tau khaws cia nyob ib ncig ntawm lub sij hawm ntev, hloov cov raj tshiab tshiab mus rau qhov muaj -80 ℃ lub freezer kom tiv thaiv degradation.
Yuav ua li cas thaw RIPA buffer?
Tom qab tshem tawm cov tshuaj ntxuav zaum kawg los ntawm cov hlwb, ntxiv cov ntim tsim nyog ntawm RIPA Buffer (1 ml ib 0.5 mus rau 5 × 107 hlwb) rau hauv lub cell pellet, thiab sib tov los yog vortex luv luv kom rov ua kom cov hlwb tag. Muab tso rau hauv dej khov lossis hauv tub yees (2–8 ° C) rau tsib feeb.
Pom zoo:
Tsis tuaj yeem tuaj koom lossis tuaj koom?
"Koj tsis tuaj yeem tuaj koom " txhais tau tias koj tuaj tsis tau. "Koj tsis tuaj yeem tuaj koom" txhais tau tias koj raug txwv tsis pub tuaj koom . Tsis tuaj yeem koom kab lus? Nws tuaj tsis tau tshoob. Hmoov tsis zoo, Jesse Eisenberg tuaj yeem tuaj koom tsis tau.
Puas plywood tuaj yeem tsis tuaj yeem?
Txawm tias cov plywood tshiab tuaj yeem warp yog tias tsis khaws cia zoo lossis yog tias tso rau hauv tshav ntuj. Ua ntej koj tso siab rau kev ntxhov siab, txawm li cas los xij, sim me ntsis kev kho dej noo. … Kev thim rov qab tuaj yeem ua tiav los ntawm kev ntxiv noo noo rau sab concave no thiab ziab lub convex humped sab .
Puas yog cov leeg ciliary yeem yeem lossis tsis yeem?
Cov leeg nqaij, uas tsis muaj kev ywj pheej, nyob hauv lub qhov muag thiab suav nrog cov leeg ciliary (saib ciliary lub cev) thiab iris. Cov leeg extrinsic, uas muaj peb khub ntawm cov leeg yeem, tau muab tso rau ntawm sclera (sab nrauv) ntawm lub qhov muag thiab tswj nws cov kev txav .
Cov qaib tsis khov puas tuaj yeem khov?
Koj tuaj yeem ua rau cov nqaij qaib nyoos thiab siav hauv lawv lub neej txee. Tseem, tsuas yog rov khov qaib nyoos uas tau muab tso rau hauv lub tub yees. Thaum kho kom raug, nws muaj kev nyab xeeb kom rov khov nqaij qaib nyoos thiab siav hauv lawv lub txee lub neej .
Koj puas tuaj yeem rov khov ntses yav tas los khov?
Tom qab ua cov zaub mov nyoos uas yav tas los khov lawm, nws muaj kev nyab xeeb rau khov cov zaub mov siav. … Tsis txhob muab cov zaub mov tso rau sab nraum lub tub yees ntev dua 2 teev. Yog tias koj yuav cov nqaij khov yav dhau los, nqaij qaib lossis ntses ntawm lub khw muag khoom, koj tuaj yeem rov khov yog tias nws tau ua haujlwm zoo Yuav ua li cas yog tias koj khov ntses ob zaug?
