Ib leeg tuaj yeem khov thiab thaw RIPA tsis pub dhau 2-3 zaug yam tsis poob nws lub zog yog tias nws khaws cia rau hauv dej khov thaum nws siv (20-30 feeb). Khaws qhov chaw tsis khov ntawm -80 degree C.
Kuv tuaj yeem khaws RIPA ntev npaum li cas?
1. Yog tias tsis siv yuav txuas ntxiv mus, nws raug pom zoo kom 10x tsis nyob ntawm 4 ° C rau 1-2 lub lis piam. Rau lub sij hawm ntev, tsis yuav tsum tau khaws cia ntawm -20 ° C. Aliquoting ntawm 10x tsis yog pom zoo yog tias yuav tsum tau ua ntau qhov kev sim me me.
Puas RIPA tsis tuaj yeem khaws cia ntawm?
Npaj RIPA tsis pub yuav tsum tau muab tso tseg thiab khaws cia ntawm −20°C. Ntxiv protease thiab/los yog phosphatase inhibitors rau thawed aliquot ua ntej siv tam sim. Ib milliliter ntawm tsis txaus yog txaus rau lyse kwv yees li 5 lab hlwb. …
Koj khaws RIPA lysates li cas?
Siv cov lysates kom sai li sai tau, thiab khaws cia kom ntev li ntev tau. Khaws lysates ntawm -80 ℃ kom ntev li ntev tau. Rau cov lysates uas yuav tsum tau khaws cia nyob ib ncig ntawm lub sij hawm ntev, hloov cov raj tshiab tshiab mus rau qhov muaj -80 ℃ lub freezer kom tiv thaiv degradation.
Yuav ua li cas thaw RIPA buffer?
Tom qab tshem tawm cov tshuaj ntxuav zaum kawg los ntawm cov hlwb, ntxiv cov ntim tsim nyog ntawm RIPA Buffer (1 ml ib 0.5 mus rau 5 × 107 hlwb) rau hauv lub cell pellet, thiab sib tov los yog vortex luv luv kom rov ua kom cov hlwb tag. Muab tso rau hauv dej khov lossis hauv tub yees (2–8 ° C) rau tsib feeb.
