Nitrocellulose daim nyias nyias yog cov matrix nrov siv rau hauv cov protein blotting vim lawv cov protein ntau-binding affinity, compatibility nrog ntau yam kev tshawb nrhiav (chemiluminescence, chromogenic, thiab fluorescence), thiab muaj peev xwm immobilize proteins, glycoproteins, los yog nucleic acids.
Vim li cas peb thiaj siv nitrocellulose?
A nitrocellulose swb, nitrocellulose membrane, lossis nitrocellulose daim ntawv yog nplaum nplaum siv rau immobilizing nucleic acids nyob rau yav qab teb blots thiab sab qaum teb blots Nws kuj yog siv rau immobilization ntawm cov protein nyob rau sab hnub poob. blots thiab atomic force microscopy rau nws nonspecific affinity rau amino acids.
Vim li cas PVDF siv rau hauv Western blotting?
Western blotting siv polyvinylidene difluoride (PVDF) daim nyias nyias yog ib qho ntawm cov txheej txheem nrov tshaj plaws rau kev kuaj pom thiab ua tus yam ntxwv ntawm cov proteins. Yog tias cov txheej txheem no tau ua ke nrog kev tiv thaiv kab mob, tus cwj pwm ntawm cov protein tshwj xeeb tuaj yeem qhia meej.
YPuas yog nitrocellulose membrane hydrophobic?
Tsis zoo li polyacrylamide, nitrocellulose muab kev txhawb nqa tsim nyog rau cov protein tom qab staining lossis immunochemical characterization. Kev khi rau nitrocellulose feem ntau yog kho los ntawm kev sib cuam tshuam hydrophobic (Schneider, 1980), txawm hais tias electrostatic rog kuj tseem koom nrog (Farrah li al., 1981.
Yuav ua li cas zoo dua nitrocellulose lossis PVDF?
Thaum nitrocellulose yog nkig thiab tsis yooj yim, PVDF yog qhov ruaj khov dua thiab muaj tshuaj tiv thaiv ntau dua ua rau nws zoo tagnrho rau kev rov ua dua thiab ua ntu zus. Nitrocellulose tuaj yeem ua pov thawj tias nyuaj rau strip thiab reprobe yam tsis muaj teeb meem poob.
