p-Nitrophenyl-beta-D-glucopyranoside yog esculinase substrate, thiab sodium desoxycholate tau hloov rau cov kua tsib ntsev. Tom qab inoculation, xim daj yog sib npaug rau xim av-dub pom nyob rau hauv 40% bile-esculin cov tshuaj tiv thaiv.
Yuav ua li cas xaiv cov khoom muaj nyob rau hauv cov kua tsib esculin test?
Bile Esculin Agar
faecium). Thawj qhov kev xaiv muaj nyob rau hauv cov agar no yog bile, uas inhibits kev loj hlob ntawm Gram-positives uas tsis yog enterococci thiab qee hom streptococci. Qhov thib ob xaiv cov khoom xyaw yog sodium azide. Cov tshuaj no inhibits kev loj hlob ntawm Gram-negatives.
Lub hom phiaj ntawm cov kua tsib esculin agar yog dab tsi?
Bile Esculin Agar yog siv feem ntau kom sib txawv Enterococcus los ntawm StreptococcusCov tswv cuab ntawm genus Enterococcus muaj peev xwm loj hlob nyob rau hauv lub xub ntiag ntawm 40% kua tsib (oxgall) thiab hydrolyzing esculin rau qabzib thiab esculetin. Esculetin ua ke nrog ferric ions los tsim ib tug complex complex.
Lub hauv paus ntsiab lus ntawm kev kuaj cov kua tsib esculin yog dab tsi?
Kev kuaj ntshav esculin yog raws li hydrolysis ntawm esculin rau hauv qabzib thiab esculetin (6, 7-dihydroxy-coumarin) los ntawm cov kab mob microorganisms uas tsim cov enzyme esculinaseEsculetin reacts nrog ib tug hlau ntsev (ferric citrate) nyob rau hauv nruab nrab los tsim ib tug phenolic hlau complex uas ua rau ib tug tsaus xim av los yog xim dub.
Kev kuaj kua tsib yog dab tsi?
Bile Solubility Test yog qhov kev sim uas txawv Streptococcus pneumoniae (zoo- soluble) los ntawm alpha-hemolytic streptococci (tsis zoo- insoluble). Streptococcus pneumoniae yog cov kua tsib soluble whereas tag nrho lwm cov alpha-hemolytic streptococci yog cov kua tsib resistant.
