Detergent ntxuav tais diav los ntawm kev tshem tawm cov rogNws ua tib yam hauv DNA rho tawm raws tu qauv, rub tawm cov rog (lipids) thiab cov proteins uas ua rau cov membranes nyob ib puag ncig ntawm tes thiab nucleus. Thaum cov membranes sib cais, DNA raug tso tawm ntawm lub cell.
Dab tsi yog cov tshuaj ntxuav tes siv rau hauv kev cais DNA?
kev cais DNA yog yooj yim nrog kev siv proteinase K enzyme, detergents, thiab chelating agents. Detergents dissolve lub cell membrane thiab denature proteins.
Lub luag haujlwm ntawm cov tshuaj ntxuav tes hauv DNA cais cov lus nug yog dab tsi?
Koj rho tawm DNA thiab kua. … Vim li cas cov tshuaj ntxuav tes ntxiv rau lub hlwb thaum lub sij hawm DNA rho tawm? Txhawm rau rhuav tshem cov phospholipid txheej. Tom qab cov hlwb raug lysed, dab tsi ntxiv rau lub cell / detergent sib tov kom precipitate DNA los ntawm kev daws?
Vim li cas cov kua ntxuav tais diav siv hauv DNA rho tawm?
xab npum muaj cov tshuaj hu ua sodium laurel sulfate uas tshem tawm cov rog thiab cov protein. … Lub tais xab npum rub tawm ntawm daim nyias nyias, tso DNAKoj tsis tuaj yeem pom DNA molecules tau vim tias lawv tau yaj hauv dej, txhais tau tias txhua tus DNA molecule nyob ib puag ncig los ntawm cov dej molecules. Dej yog ib tug polar molecule heev.
Lub hom phiaj ntawm cov tshuaj ntxuav tes hauv cov tshuaj lysis yog dab tsi?
Hauv kev tshawb fawb txog roj ntsha, cov tshuaj ntxuav tes yog siv los ua cov lyse hlwb (tso cov proteins soluble), solubilize membrane proteins thiab lipids, tswj cov protein crystallization, tiv thaiv tsis muaj kev sib txuas hauv affinity purification thiab immunoassay cov txheej txheem, thiab siv los ua additives hauv electrophoresis.
