Metagenomics txhais tau tias yog qhov kev tshuaj ntsuam genetic ncaj qha ntawm cov genomes uas muaj cov qauv ib puag ncig Lub teb pib pib nrog lub cloning ntawm ib puag ncig DNA, ua raws li kev tshuaj ntsuam xyuas kev ua haujlwm [1], thiab tom qab ntawd tau ua tiav sai sai los ntawm kev tua phom ncaj qha ntawm ib puag ncig DNA [2, 3].
Tus txheej txheem ntawm metagenomics yog dab tsi?
Metagenomics koom nrog kev tau txais DNA los ntawm txhua yam kab mob hauv zej zog, yam tsis tas yuav txheeb xyuas txhua hom kev koom nrog. Tom qab cov noob tau ua tiav thiab muab piv nrog cov txheej txheem txheeb xyuas, cov haujlwm ntawm cov noob no tuaj yeem txiav txim siab.
Yuav siv cov txheej txheem dab tsi hauv metaagenomics?
Ib qho piv txwv ntawm cov zej zog microbial yog sau los ntawm cov dej hiav txwv, cov xuab zeb, cov tsiaj nyeg, lossis lwm qhov chaw nyob hauv hiav txwv. Tom qab ntawd cov hlwb uas sau tau yog lysed thiab DNA tau muab rho tawm los ntawm lawv cov lysate. Cov txheej txheem uas ua rau metaagenomics ua tau yog phom sib luag, uas ua raws cov cheeb tsam random ntawm DNA nyob rau hauv ib qho kev tsis sib haum xeeb.
3 kauj ruam rau kev kawm metagenomics yog dab tsi?
Cov kauj ruam thiab txheej txheem ntawm metaagenomics:
- Sample collection:
- DNA rho tawm:
- Sample npaj:
- Sample analysis:
Metagenomics ua haujlwm li cas?
Kev ua haujlwm metagenomics suav nrog cais DNA los ntawm cov zej zog microbial los kawm txog kev ua haujlwm ntawm cov proteins encoded. Nws koom nrog cloning DNA tawg, qhia cov noob hauv tus tswv tsev, thiab tshuaj xyuas cov dej num enzymatic.
