Uncompetitive inhibitors Uncompetitive inhibitors Uncompetitive inhibitors yuav txo ob leeg vmax thiab pom meej Km los ntawm tib yam [v max, i=vmax/(1 + C i/Ki), and Km, app=Km/(1 + Ci/ /Ki) Qhov txo qis hauv Km tshwm sim los ntawm cov tshuaj tiv thaiv ES + I ⇔ ESI, uas txo cov tus nqi ntawm ES thiab ua rau E + S ⇔ ES equilibrium hloov mus rau sab xis. https://www.sciencedirect.com › uncompetitive-inhibitor
Uncompetitive Inhibitor - tus txheej txheem cej luam | ScienceDirect Topics
khi tsuas yog rau cov enzyme-substrate complex, tsis yog rau cov enzyme dawb, thiab lawv txo kcat thiab Km(qhov txo qis hauv Km stems los ntawm qhov tseeb tias lawv muaj rub tawm. Lub kaw lus deb ntawm cov enzyme dawb mus rau enzyme-substrate complex).
Vim li cas ho tsis sib tw inhibitors tsis cuam tshuam Km?
Thaum tus tsis sib tw inhibitor ntxiv Vmax hloov, thaum Km tseem tsis hloov. … Hauv kev txwv tsis pub muaj kev sib tw, tus inhibitor khi rau ib qho chaw allosteric thiab tiv thaiv cov enzyme-substrate complex los ntawm kev ua cov tshuaj tiv thaivQhov no tsis cuam tshuam rau Km (affinity) ntawm cov enzyme (rau lub substrate).
Puas kev sib tw inhibitors cuam tshuam Km?
Kev sib tw inhibitors tuaj yeem khi rau E thiab tsis yog ES. Lawv nce Km los ntawm kev cuam tshuamnrog kev khi ntawm cov substrate, tab sis lawv tsis cuam tshuam Vmax vim tias tus inhibitor tsis hloov cov catalysis hauv ES vim nws tsis tuaj yeem khi rau ES.
Yuav ua li cas irreversible inhibitors cuam tshuam Km thiab Vmax?
Yog tias qhov concentration ntawm irreversible inhibitor tsawg dua qhov concentration ntawm enzyme, tus inhibitor irreversible yuav tsis cuam tshuam Km thiab yuav txo Vmax. Yog hais tias qhov concentration ntawm irreversible inhibitor yog ntau tshaj qhov concentration ntawm enzyme, tsis muaj catalysis yuav tshwm sim.
Yuav ua li cas rau Vmax thiab Km hauv kev txwv tsis pub muaj kev sib tw?
Ib yam thib peb ntawm enzymatic inhibition yog qhov tsis sib tw inhibition, uas muaj cov khoom khib nyiab ntawm qhov txo qis Vmax thiab txo Km. … Yog li, paradoxically, uncompetitive inhibition ob qho tib si txo Vmax thiab nce ib enzyme affinity rau nws substrate.
