Ib primer yog luv luv nucleic acid ib ntus uas muab qhov pib rau DNA synthesis. Lub synthesis ntawm ib tug primer yog tsim nyog vim hais tias cov enzymes uas synthesize DNA synthesis DNA DNA synthesis yog cov biological txheej txheem uas ib tug deoxyribonucleic acid (DNA) molecule yog tsim nyob rau hauv lub cell, txhua ntawm ob. strands ntawm DNA molecule ua raws li ib tug qauv rau cov synthesis ntawm ib tug complementary strand. https://www.nature.com › subjects › dna-synthesis
DNA synthesis - Kev tshawb fawb tshiab thiab xov xwm | Nature
uas yog hu ua DNA polymerases, tsuas yog xa cov DNA nucleotides tshiab rau cov kab uas twb muaj lawm ntawm nucleotides. …
Dab tsi yog primer rau DNA polymerase?
Primer. Lub primer yog luv, ib leeg-stranded DNA ib ntus siv hauv cov txheej txheem polymerase saw tshuaj tiv thaiv (PCR). Nyob rau hauv txoj kev PCR, ib khub ntawm primers yog siv los hybridize nrog cov qauv DNA thiab txhais cov cheeb tsam ntawm DNA uas yuav tsum tau amplified. Primers kuj raug hu ua oligonucleotides.
Vim li cas DNA polymerase xav tau primer tab sis RNA polymerase tsis?
Tsis paub DNA polymerase tuaj yeem pib cov saw tshiab(de novo). DNA polymerase tuaj yeem ntxiv cov nucleotide rau tsuas yog ib pawg 3'-OH, thiab, yog li ntawd, xav tau cov primer uas nws tuaj yeem ntxiv thawj nucleotide.
Vim li cas DNA polymerase xav tau ob primers?
Ob tus primers yog siv nyob rau hauv txhua qhov tshuaj tiv thaiv PCR, thiab lawv tau tsim kom lawv flank lub hom phiaj cheeb tsam (cheeb tsam uas yuav tsum tau theej) Qhov ntawd yog, lawv tau muab cov kab ke uas yuav ua rau lawv khi rau qhov sib txawv ntawm cov qauv DNA, tsuas yog nyob ntawm ntug ntawm thaj av kom tau theej.
DNA pol ua haujlwm yam tsis muaj primer?
Zaj. DNA polymerases tsis tuaj yeem pib synthesis ntawm DNA chains hauv qhov tsis tuaj ntawm cov khoom siv priming. Txawm hais tias DNA polymerases tuaj yeem txuas ntxiv luv luv DNA primers uas yog randomly annealed rau lub template strand hauv vitro, tshwj xeeb priming yog pom nyob rau hauv vivo.
