Yeast Episomal plasmids (YEp): Cov no feem ntau zoo ib yam li cov kab mob plasmids thiab yog xav tias "siab luam". Ib qho tawg ntawm 2 micron lub voj voog (lub ntuj poov xab plasmid) tso cai rau 50+ cov ntawv luam kom ruaj khov rau ib lub xov tooj.
Yeast vector yog dab tsi?
Cov kab mob poov xab yog siv hauv molecular biology los qhia DNA ntawm kev txaus siab rau cov poov xab rau cov protein ntau thiab kev qhia. Feem ntau siv cov poov xab qhia vectors suav nrog Saccharomyces cerevisiae thiab Pichia pastoris.
cov poov xab plasmids nyob qhov twg?
Ib qho nthuav poov xab plasmid hu ua 2u lub voj voog. Lub voj voog 2u yog lub voj voog 6.3 kb, cov ntsiab lus extrachromosomal pom nyob rau hauv lub nucleus ntawm feem ntau Saccharomyces cerevisiae hom.
Kev sib koom ua ke Episomal yog dab tsi?
Episomal plasmids muaj ob lub ntsiab lus; CEN6-ARSH4-HIS3 ib ntus los ntawm cov poov xab rau kev saib xyuas ntawm plasmid raws li ib qho kev ywj pheej ntawm cov hlwb thiab E. coli oriT (keeb kwm ntawm kev hloov pauv) cov noob rau kev sib txuas sib haum xeeb hloov ntawm plasmid los ntawm cov kab mob mus rau tus tswv tsev.
CEN plasmid yog dab tsi?
koj tuaj yeem hloov pauv cov poov xab nrog ntau qhov sib txawv plasmids (zoo tshaj ib qho tom qab). Nyob ib sab ntawm tus cim, cov plasmids no muaj "ARS" (autonomously replicating sequence) thiab feem ntau yog "CEN" (poov tshuaj centromere); lawv rov ua dua li chromosomes thiab lawv cov lej luam tau tswj hwm (qhov nruab nrab 1 plasmid ib lub xov tooj ntawm tes).
