DNA replication pib ntawm lub hauv paus pib ntawm replication. Tseem tsuas yog ib qho keeb kwm hauv prokaryotes (hauv E. coli, oriC) thiab nws yog tus yam ntxwv los ntawm cov arrays ntawm rov ua ntu zus.
Puas prokaryotes muaj ib qho keeb kwm ntawm kev rov ua dua?
Nyob rau hauv cov hlwb prokaryotic, tsuas muaj ib qho chaw ntawm keeb kwm, kev rov ua dua tshwm sim nyob rau hauv ob qho kev tawm tsam tib lub sijhawm, thiab tshwm sim hauv cell cytoplasm. Eukaryotic hlwb ntawm qhov tod tes, muaj ntau lub ntsiab lus ntawm keeb kwm, thiab siv unidirectional replication nyob rau hauv lub nucleus ntawm lub cell.
Vim li cas prokaryotes tsuas muaj ib lub hauv paus ntawm kev rov ua dua?
Nyob rau hauv prokaryotic genome, tib lub hauv paus chiv keeb ntawm replication muaj ntau A-T puag khub, uas muaj tsis muaj zog hydrogen bonding dua G-C puag khub, thiab ua kom yooj yim rau DNA strands rau cais. Ib qho enzyme hu ua helicase unwinds DNA los ntawm kev rhuav tshem cov hydrogen bonds ntawm cov nitrogenous puag khub.
Muaj pes tsawg lub hauv paus ntawm kev rov ua dua pom hauv prokaryotes?
Prokaryotic genomes muaj ib lossis ob peb chromosomes [1], feem ntau yog ncig [2]. Cov chromosomes muaj ob txoj kab sib luag DNA sib luag, thiab yuav tsum muaj ib lub hauv paus ntawm kev rov ua dua(eubacteria) [3] lossis tej zaum yuav muaj ib zaug lossis ntau lub hauv paus (archaea) [4].
Puas prokaryotes thiab eukaryotes muaj tib lub hauv paus chiv keeb ntawm DNA replication?
Ob leeg eukaryotic thiab prokaryotic DNA polymerases tsim tawm RNA primers ua los ntawm primase. Eukaryotic DNA replication yuav tsum tau muaj ntau yam replication diav rawg, thaum prokaryotic replication siv ib lub hauv paus chiv keebkom sai replicate tag nrho genome.
