Kev ntxhov siab Oxidative tuaj yeem ntsuas tsis ncaj los ntawm ntsuas qib DNA / RNA kev puas tsuaj, lipid peroxidation, thiab protein oxidation / nitration, es tsis yog ntsuas ncaj qha ntawm cov pa oxygen reactive. Cov cim oxidative kev ntxhov siab no muaj kev ruaj khov dua li cov pa oxygen reactive.
Koj ua li cas kuaj rau oxidative stress?
Lub xub ntiag ntawm oxidative kev nyuaj siab yuav raug sim nyob rau hauv ib qho ntawm peb txoj kev: (1) ntsuas ncaj qha ntawm ROS; (2) Kev ntsuas qhov tshwm sim ntawm kev puas tsuaj rau biomolecules; thiab (3) nrhiav pom cov tshuaj antioxidant.
Qhov ntsuas ntawm oxidative kev nyuaj siab yog dab tsi?
Kev tsim cov superoxide radical (O2(•-)) tau ntsuas raws li qhov ntsuas ntawm cov pa oxygen reactive ntau lawm; lipid peroxidation (TBARS) thiab cov qib protein carbonyl tau suav tias yog cov cim ntawm kev puas tsuaj oxidative, thiab kev ua haujlwm ntawm antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione …
Dab tsi yog oxidative stress biomarkers?
Biomarkers ntawm oxidative stress. Biomarkers ntawm oxidative kev nyuaj siab tuaj yeem raug cais raws li molecules uas tau hloov kho los ntawm kev cuam tshuam nrog ROS hauv microenvironment; thiab cov molecules ntawm cov kab mob antioxidant uas hloov pauv hauv cov lus teb rau kev ntxhov siab redox.
Koj ntsuas ROS li cas?
Cov qib intracellular ntawm ROS tuaj yeem ntsuas tau los ntawm flow cytometry siv dihydrofluorescein diacetate (DCFH) txhawm rau txheeb xyuas cov kab mob hauv cov cellular hydrogen peroxide radicals. Cov xim no yog oxidized rau cov fluorescent derivative dichlorofluorescein (DCF), uas tau kuaj pom los ntawm kev siv cov flow cytometer [46, 47, 48, 82].
