ər/ lossis VP yog ib qho kev sim siv los kuaj xyuas acetoin hauv cov kab mob kab mob kab mob. Qhov kev sim no yog ua los ntawm kev ntxiv alpha-naphthol thiab potassium hydroxide rau Voges-Proskauer broth uas tau raug inoculated nrog cov kab mob. Cov xim liab cherry qhia tau tias muaj txiaj ntsig zoo, thaum xim daj-xim av qhia qhov tshwm sim tsis zoo.
Kev ntsuas dab tsi yog siv rau lactose fermentation?
MacConkey agar feem ntau yog siv los sib txawv ntawm Enterobacteriaceae. Cov kab mob ntawm sab laug yog qhov zoo rau lactose fermentation thiab ntawm sab xis yog qhov tsis zoo.
Koj ua li cas MRVP xeem?
Yuav kom sim txoj kev no, ib qho aliquot ntawm MR/VP kab lis kev cai raug tshem tawm thiab a-naphthol thiab KOH are ntxiv. Lawv tau shaken ua ke thiab muab tso tseg li ntawm ib teev kom txog thaum cov txiaj ntsig tuaj yeem nyeem tau. Kev kuaj Voges-Proskauer kuaj pom muaj acetoin, ua ntej ntawm 2, 3 butanediol.
VP xeem yog dab tsi?
Voges-Proskauer (VP) kev sim yog siv los txiav txim siab seb lub cev puas tsim acetylmethyl carbinol los ntawm cov piam thaj fermentation. Yog tias tam sim no, acetylmethyl carbinol hloov mus rau diacetyl nyob rau hauv muaj ∝- naphthol, muaj zog alkali (40% KOH), thiab atmospheric oxygen.
Koj ua li cas Voges Proskauer xeem?
txheej txheem ntawm Voges Proskauer Test
- Txhim kho lub raj ntawm MR/VP kua zaub nrog cov kab lis kev cai ntshiab ntawm cov kab mob kuaj.
- Cub rau 24 teev ntawm 35 ° C.
- Thaum kawg ntawm lub sijhawm no, aliquot 1 mL ntawm broth mus rau lub raj huv huv.
- Ntxiv 0.6mL ntawm 5% α-naphthol, ua raws li 0.2 mL ntawm 40% KOH.
