Qhov ntsuas kub zoo tshaj plaws thiab pH rau trypsin yog 65 ° C thiab pH 9.0, feem.
Ntawm li cas pH yog trypsin nquag tshaj?
Trypsin yog ib qho serine protease uas zais los ntawm pancreas thiab ua haujlwm tshaj plaws hauv pH ntau nruab nrab ntawm 7 thiab 9 ntawm 37°C.
Ntawm pH ua trypsin denature?
Peb cov kev tshawb fawb hauv vitro kuj tau qhia tias trypsin tau denatured qeeb ntawm pH 6 thiab 4.25 thiab nrawm ntawm 4.25 thiab 3.75. Tus nqi ntawm denaturation tau nrawm dua nyob rau hauv chav tsev kub thiab qeeb hauv cov dej khov dua li qhov dav ntawm pHs.
Ntawm pH dab tsi ua qhov enzyme no ua haujlwm zoo tshaj?
Enzymes hauv plab, xws li pepsin (uas zom cov protein), ua haujlwm zoo tshaj plaws hauv cov kua qaub (pH 1 - 2), tab sis feem ntau cov enzymes hauv lub cev ua haujlwm zoo tshaj pH 7.
Yuav ua li cas yog tias pH qis dhau rau ib qho enzyme?
Ntawm qhov pH qis heev, qhov cuam tshuam no ua rau cov protein nthuav tawm, cov duab ntawm qhov chaw ua haujlwm tsis muaj ntxiv rau cov substrate molecule thiab cov tshuaj tiv thaiv tsis tuaj yeem ua tau ntxiv lawm catalysed los ntawm enzyme. Lub enzyme tau denatured.