Q: Hauv TLC, lub hom phiaj ntawm kev siv cov kuab tshuaj eluent yog dab tsi? Cov ntsiab lus yog theem mobile hauv chromatography, piv txwv li, cov kuab tshuaj hauv lub tank tsim. Thaum lub sij hawm txoj kev loj hlob ntawm lub chromatogram, lub eluent faib cov qauv koj pauv mus rau lub TLC phaj tshaj lub adsorbent ntawm lub phaj.
Lub hom phiaj ntawm qhov hnyav pem hauv ntej hauv chromatography yog dab tsi?
Hauv daim ntawv chromatography, ntub dej txav ntug ntawm cov kuab tshuaj uas nce mus raws qhov chaw uas qhov sib cais ntawm qhov sib tov tshwm sim.
TLC ua dab tsi?
Eluent: cov kuab tshuaj lossis sib xyaw ntawm cov kuab tshuaj (theem txawb) siv los tsim TLC chromatogram (phaj). Elution: tag nrho cov txheej txheem ntawm kev tsim TLC phaj.
Dab tsi yog qhov hnyav hauv TLC?
Rov qab-theem hom Hauv kev hloov pauv-theem chromatography, cov txheej txheem hnyav yog:
Cov dej sib xyaw los yog cov dej tsis muaj dej thiab cov dej miscible organic solvents xws li acetonitrile (ACN), methanol, thiab tetrahydrofuran (THF) Lwm cov kuab tshuaj siv tau xws li ethanol (EtOH) & isopropanol (IPA).
Vim li cas cov kuab tshuaj tseem ceeb hauv TLC?
Cov kuab tshuaj, uas nyob hauv qab ntawm lub thawv, taug kev mus txog txheej ntawm adsorbent los ntawm kev ua haujlwm capillary, hla qhov chaw thiab, thaum nws txuas ntxiv mus, txav mus rau qhov chaw. cov tebchaw nyob rau hauv qhov sib xyaw ua ke ntawm cov phaj sib txawv uas ua rau muaj kev sib cais ntawm cov tebchaw.
