Diphenylamine method Lwm txoj kev nqus raws li kev ntsuas DNA siv diphenylamine. Diphenylamine reacts nrog deoxyribose qab zib nyob rau hauv acidic tej yam kev mob thiab tsim ib tug xiav complex uas yuav tsum tau kom muaj nuj nqis ntawm 595 nm.
Yuav ua li cas reagent siv los ntsuas DNA?
Fluorescence detection nrog Hoechst 33258 reagent yog ib txoj hauv kev rhiab thiab meej rau DNA kom muaj nuj nqis yog tias G + C cov ntsiab lus tsawg dua 50%. Tsis tas li ntawd, txoj kev no tso cai rau kom muaj nuj nqis ntawm DNA tsawg heev (nanogram nplai).
DNA yog dab tsi?
DNA kom muaj nuj nqis thiab RNA kom muaj nuj nqis, feem ntau hu ua nucleic acid kom muaj nuj nqis, feem ntau ua los txiav txim siab qhov nruab nrab ntawm DNA lossis RNA hauv ib qho qauv ua ntej ua ntej kev sim qis..
Txoj kev twg yog siv rau kev kwv yees ntawm DNA?
Yuav kwv yees tus nqi ntawm DNA tam sim no hauv cov tshuaj tsis paub los ntawm diphenylamine method PRINCIPLE: Thaum DNA tau kho nrog diphenylamine nyob rau hauv cov kua qaub ib qho xim ntsuab ntsuab yog tsim uas Nws muaj qhov nqus siab tshaj plaws ntawm 595nm. Cov tshuaj tiv thaiv no yog muab los ntawm 2 deoxypentose feem ntau.
Yuav ua li cas yog txoj kev zoo tshaj plaws rau DNA kom muaj nuj nqis?
Xaiv qhov zoo tshaj plaws DNA Quantification Method
- Real-Time Polymerase Chain Reaction. Real-time PCR, qee zaum hu ua qPCR, yog ib qho ntawm RNA thiab DNA quantification txoj kev siv niaj hnub no, vim nws qhov rhiab heev, qhov tshwj xeeb, thiab ntau yam muaj zog. …
- YUV-Vis Spectrophotometric. …
- Fluorescence.
