Ib txoj hauv kev kuaj uas siv antibodies txhawm rau kuaj xyuas qee yam antigens (cov cim) hauv cov qauv ntawm cov ntaub so ntswg. Cov tshuaj tiv thaiv kab mob feem ntau yog txuas mus rau ib qho enzyme lossis fluorescent dye. Tom qab cov tshuaj tiv thaiv khi rau cov tshuaj tiv thaiv hauv cov qauv ntaub so ntswg, cov enzyme los yog cov zas xim tau qhib, thiab cov tshuaj tiv thaiv tuaj yeem pom hauv lub tshuab ntsuas.
IHC kuaj tau li cas?
Immunohistochemical (IHC) lossis immunoperoxidase stains yog lwm pab pawg ntawm cov kev kuaj tshwj xeeb. Lub hauv paus ntsiab lus ntawm txoj kev no yog ib qho tshuaj tiv thaiv kab mob hu ua cov tshuaj tiv thaiv kab mob yuav xa nws tus kheej mus rau qee yam tshuaj, hu ua antigens, uas nyob rau hauv lossis hauv lub cell
Thaum twg koj ua immunohistochemistry?
Immunohistochemistry (IHC) yog ib qho tseem ceeb ntawm kev siv monoclonal nrog rau cov tshuaj tiv thaiv polyclonal los txiav txim cov ntaub so ntswg ntawm cov tshuaj tiv thaiv kev txaus siab rau kev noj qab haus huv thiab kab mob. IHC yog dav siv rau kev kuaj mob qog noj ntshav; Cov qog nqaij hlav tshwj xeeb yog qhia tawm de novo los yog tswj hwm hauv qee yam qog nqaij hlav.
IHC lub hauv paus ntsiab lus yog dab tsi?
Taw qhia. Immunohistochemistry (IHC) yog ib txoj hauv kev txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau cov kab mob hauv cov ntaub so ntswg. sib txawv.
Koj xav tau dab tsi rau immunohistochemistry?
Ib txoj kab ke immunohistochemistry dav dav muaj plaub kauj ruam tseem ceeb:
- Kev kho-kom khaws txhua yam hauv nws qhov chaw.
- Antigen retrieval-kom muaj protein ntau rau kev kuaj pom.
- Thaiv-kom txo qis pesky keeb kwm yav dhau.
- Antibody labeling and visualization-kom tau cov duab zoo nkauj.