Homology qhia kho yog lub tshuab hauv cov hlwb los kho cov kab mob DNA ob npaug. Daim ntawv tshaj plaws ntawm HDR yog homologous recombination. Lub tshuab HDR tsuas tuaj yeem siv los ntawm lub xovtooj ntawm tes thaum muaj ib qho homologous DNA tam sim no nyob rau hauv lub nucleus, feem ntau hauv G2 thiab S theem ntawm lub voj voog ntawm tes.
homology qhia txog kev kho li cas?
Homology qhia kho (HDR) yog ib lub tshuab hauv hlwb los kho ob-strand DNA lesions … Lwm yam piv txwv ntawm homology-qhia kho muaj xws li ib leeg-strand annealing thiab breakage-induced rov ua dua. Thaum tsis muaj DNA homologous, lwm cov txheej txheem hu ua non-homologous kawg koom nrog (NHEJ) yuav tshwm sim.
Kev kho homology qhia hauv Crispr ua haujlwm li cas?
Cov kab ntxeem tau tuaj yeem hloov pauv ib txoj hlua ntawm homologous DNA duplex thiab ua khub nrog lwm tus; Qhov no tshwm sim hauv kev tsim cov hybrid DNA hu ua lub voj xaav (D voj). Qhov no yog lub ntsiab lus txhais ntawm HDR. Cov intermediates recombination tuaj yeem ces tau txiav txim siab kom ua tiav cov txheej txheem kho DNA.
Dab tsi yog qhov sib txawv ntawm kev sib koom ua ke tsis sib xws thiab kev kho homology?
Dab tsi yog qhov txawv ntawm qhov tsis sib xws ntawm qhov kawg koom nrog (NHEJ) thiab homology-directed kho (HDR)? … Ntawm nws qhov tseem ceeb, NHEJ-tawg kawg tuaj yeem ligated yam tsis muaj homologous template, whereas HDR-breaks yuav tsum tau ib tug qauv los qhia kev kho. NHEJ yog qhov kho tau zoo heev uas muaj kev ua haujlwm tshaj plaws hauv lub xovtooj.
Kev kho homology yuav siv sijhawm ntev npaum li cas?
Ntawm no, peb siv tib lub tswv yim los tshawb txog cov tshuaj tiv thaiv kev kho mob ntawm homology-qhia kev kho siv ib leeg-stranded DNA pub nrog ob txhais caj npab ntawm homology mus rau qhov chaw. Lub kaw lus tau piav qhia hauv daim duab 1 thiab tag nrho cov tshuaj tiv thaiv yuav siv sijhawm ~16 h kom tiav.
Homology-dependent double strand break repair
