Protoplast fusion yog ua tiav los ntawm kev sib ntxiv ntawm PEG nrog siab concentration ntawm calcium ntawm pH ntawm 8-10 thiab los ntawm electrofusion (Olivares-Fuster li al., 2005). Somatic hybrids yog tsim los ntawm fusion ntawm nuclei thiab cytoplasm ntawm ob hom.
Koj ua li cas fuse protoplasts?
Cov txheej txheem somatic fusion tshwm sim hauv plaub kauj ruam:
- Kev tshem tawm ntawm phab ntsa ntawm tes ntawm ib lub xovtooj ntawm txhua hom nroj tsuag uas siv cellulase enzyme los tsim cov cell somatic hu ua protoplast.
- Cov hlwb ces fused siv hluav taws xob (electrofusion) los yog tshuaj kho mob los koom lub hlwb thiab fuse ua ke lub nuclei.
Dab tsi yog cov kauj ruam hauv somatic hybridization?
Cov kauj ruam tseem ceeb hauv cov txheej txheem ntawm kev sib xyaw somatic yog: (1) kev sib cais ntawm protoplasts, (2) fusion ntawm protoplasts, (3) kab lis kev cai ntawm protoplasts los tsa tag nrho cov nroj tsuag, (4) kev xaiv ntawm hybrid hlwb thiab kev txheeb xyuas hybridity Page 5 ISOLATION OF PROTOPLAST Protoplast tuaj yeem raug cais tawm ntawm yuav luag txhua qhov chaw cog xws li …
Yuav ua li cas hybrid protoplasts tau txheeb xyuas hauv somatic hybridization?
Somatic hybridization yog cov txheej txheem uas tso cai rau kev tswj hwm ntawm cov cellular genome los ntawm cov txheej txheem hu ua protoplast fusion. Nws yog hom kev hloov kho caj ces hauv cov nroj tsuag uas ob hom ntawm cov nroj tsuag sib txawv ua ke rautsim cov nroj tsuag tshiab nrog cov yam ntxwv ntawm ob qho tib si.
Dab tsi ua rau fusion ntawm protoplast hauv somatic hybridization?
Protoplast fusion yuav raug ntxias los ntawm hluav taws xob los yog tshuaj, los ntawm ntxiv ntawm 20-40% polyethylene glycoluas ua rau protoplasts aggregation thiab dilution ntawm polyethylene glycol uas ua rau protoplast fusion. Lub somatic hybrid yuav tsum raug xaiv tom qab fusion ntawm protoplasts.
